Nitrate reductase (NR)-dependent NO production mediates ABA- and H2O2-induced antioxidant enzymes
Nitrate reductase (NR)-dependent NO production mediates ABA- and H2O2-induced antioxidant enzymes
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硝酸还原酶 (NR) 依赖性 NO 产生介导 ABA 和 H2O2 诱导的抗氧化酶
DOI：
10.1016/j.plaphy.2013.10.030
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发表时间：
2014-01-01
影响因子：
6.5
通讯作者：
Guo, Zhenfei
中科院分区：
文献类型：
--
作者：
Lu, Shaoyun;Zhuo, Chunliu;Guo, Zhenfei
脱落酸 (ABA)、H2O2 和一氧化氮 (NO) 是植物适应环境胁迫过程中基因表达和生理反应的重要信号。与野生型相比，使用过表达柱花草 9-顺式环氧类胡萝卜素双加氧酶基因 (SgNCED1) 的转基因烟草植物来研究 NR 衍生的 NO 产生在 ABA 和 H2O2 诱导的抗氧化酶中的重要作用，以提高 ABA 水平，或过表达小麦草酸氧化酶基因 (OxO) 以提高 H2O2 水平。与野生型相比，所有转基因植物中均观察到较高水平的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和硝酸还原酶（NR）活性以及NO产生。为了研究 ABA、H2O2 和 NR 产生的 NO 在诱导抗氧化酶活性中的关系，实验中使用了 ABA 生物合成抑制剂、H2O2 和 NO 清除剂以及 NR 抑制剂。结果表明，OxO 转基因植物中 H2O2 诱导的 SOD、CAT 和 APX 活性依赖于 NR 衍生的 NO，而 SgNCED1 转基因植物中 ABA 诱导的活性依赖于 H2O2 和 NR 衍生的 NO。与未改变的硝酸还原酶 2 (NIA2) 相比，NIA1 转录在两种类型的转基因植物中均被诱导。这表明 NR 衍生的 NO 对于 ABA 或 H2O2 诱导的抗氧化酶活性至关重要。 (C) 2013 Elsevier Masson SAS。版权所有。
Abscisic acid (ABA), H2O2 and nitric oxide (NO) are important signals in gene expression and physiological responses during plant adaptation to environmental stresses. The essential role of NR-derived NO production in ABA and H2O2 induced antioxidant enzymes were studied using transgenic tobacco plants over-expressing Stylosanthes guianensis 9-cis-epoxycartenoid dioxygenase gene (SgNCED1) for elevated ABA level, or over-expressing wheat oxalate oxidase gene (OxO) for elevated H2O2 level in comparison to the wild type. Compared to the wild type, higher levels of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and nitrate reductase (NR) activities and NO production were observed in all transgenic plants. For investigating the relationship of ABA, H2O2, and NR-produced NO in the induction of antioxidant enzyme activities, an inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and an inhibitor of NR were used in the experiments. The results indicate that H2O2-induced activities of SOD, CAT, and APX depends on NR-derived NO in OxO transgenic plants, while ABA-induced activities depends on H2O2 and NR-derived NO in SgNCED1 transgenic plants. Compared to unaltered nitrate reductase 2 (NIA2), NIA1 transcript was induced in both types of transgenic plants. It is suggested NR-derived NO is essential for ABA- or H2O2-induced antioxidant enzyme activities. (C) 2013 Elsevier Masson SAS. All rights reserved.