Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes.
Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes.
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DOI：
10.1016/j.fsi.2022.04.050
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发表时间：
2022-05
影响因子：
4.7
通讯作者：
Meng Li;Qian Huang;Xiaoyang Lv;H. J. Small;Caiwen Li
中科院分区：
文献类型：
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作者：
Meng Li;Qian Huang;Xiaoyang Lv;H. J. Small;Caiwen Li
血甲藻属（Hematodinium）的寄生性甲藻对世界各地多种具有商业价值的海洋甲壳类造成了巨大的经济损失。最近为更好地了解寄生虫的生命周期和生物学所做的努力提高了我们对疾病生态学的理解。然而，关于宿主-寄生虫相互作用，特别是血甲藻如何逃避宿主免疫反应的研究还很缺乏。为了解决这一不足，我们使用了全面的组学方法（miRNA转录组学，基于iTRAQ的蛋白质组学）来深入了解本研究中三疣梭子蟹和血甲藻血细胞之间的宿主-寄生虫相互作用。寄生甲藻H.重组人血细胞的miRNome和蛋白质组，在转录后和翻译水平调节宿主免疫应答，并引起对宿主免疫应答的转录后调节。多种重要的细胞和体液免疫相关途径（例如，细胞凋亡、细胞内吞、ECM-受体相互作用、proPO激活途径、Toll样信号通路、Jak-STAT信号通路）均受到血吸虫的显著影响。通过调节宿主miRNome，显著抑制了宿主的免疫应答，包括促红细胞生成、促红细胞生成素活化和抗菌肽。通过吞噬体和过氧化物酶体途径的转录后失调，细胞内稳态不平衡。ECM-受体相互作用和粘着斑途径的转录后下调严重影响了细胞结构和通讯。最后，H.该寄生虫可引起甲壳动物血细胞miRNome和蛋白质组的显著变化，在甲壳动物宿主中表现出多方面的免疫调节作用和潜在的免疫抑制机制。
Parasitic dinoflagellates in genusHematodiniumhave caused substantial economic losses to multiple commercially valuable marine crustaceans around the world. Recent efforts to better understand the life cycle and biology of the parasite have improved our understanding of the disease ecology. However, studies on the host-parasite interaction, especially howHematodiniumparasites evade the host immune response are lacking. To address this shortfall, we used the comprehensive omics approaches (miRNA transcriptomics, iTRAQ-based proteomics) to get insights into the host-parasite interaction between hemocytes fromPortunus trituberculatusandHematodinium pereziin the present study. The parasitic dinoflagellateH. pereziremodeled the miRNome and proteome of hemocytes from challenged hosts, modulated the host immune response at both post-transcriptional and translational levels and caused post-transcriptional regulation to the host immune response. Multiple important cellular and humoral immune-related pathways (ex. Apoptosis, Endocytosis, ECM-receptor interaction, proPO activation pathway, Toll-like signaling pathway, Jak-STAT signaling pathway) were significantly affected byHematodiniumparasites. Through modulation of the host miRNome, the host immune responses of nodulation, proPO activation and antimicrobial peptides were significantly suppressed. Cellular homeostasis was imbalanced via post-transcriptional dysregulation of the phagosome and peroxisome pathways. Cellular structure and communication was seriously impacted by post-transcriptional downregulation of ECM-receptor interaction and focal adhesion pathways. In conclusion,H. pereziparasites could trigger striking changes in the miRNome and proteome of crustacean hemocytes, and this parasite exhibited multifaceted immunomodulatory effects and potential immune-suppressive mechanisms in crustacean hosts.