Translational misreading: mutations in translation elongation factor 1alpha differentially affect programmed ribosomal frameshifting and drug sensitivity.
Translational misreading: mutations in translation elongation factor 1alpha differentially affect programmed ribosomal frameshifting and drug sensitivity.
复制标题
翻译误读：翻译延伸因子 1α 的突变对程序化核糖体移码和药物敏感性有不同的影响。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Kinzy
中科院分区：
文献类型：
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作者：
J. Dinman;T. Kinzy
翻译延长因子1α(EF-1α)催化将氨酰tRNAs运送到延长的核糖体的关键步骤。一系列含有编码EF-1α的TEF2基因突变等位基因的酿酒酵母菌株的表型与与翻译相关的细胞过程的影响一致。这些缺陷包括(1)条件性生长缺陷，(2)抗生素敏感性或耐药性，(3)+1或-1核糖体移框效率改变，以及(4)杀手表型保持改变。虽然所有突变的等位基因都被分离为显性的+1移码抑制基因，但当这些突变作为EF-1α的唯一形式存在时，对细胞的影响是非常不同的。观察到等位基因特异性效应是关于它们改变程序性+1移码的效率的能力，而不是相对于程序性-1核糖体移码的效率。在含有TEF2-4和TEF2-9突变等位基因的菌株中，-1移码的效率显著改变，这进一步与保持L-A的杀手表型和M1卫星病毒的能力降低有关，M1型卫星病毒是体内翻译保真度的测试。根据所提出的关于+1或-1核糖体移码所需的不同A-和P-位占据状态的模型，这些结果有助于分析EF-1α与翻译装置之间的相互作用。
The translation elongation feactor 1alpha (EF-1alpha) catalyzes the critical step of delivering aminoacyl-tRNAs to the elongating ribosome. A series of Saccharomyces cerevisiae strains containing mutant alleles of the TEF2 gene encoding EF-1alpha have phenotypes consistent with effects on cellular processes related to translation. These include (1) conditional growth defects, (2) antibiotic sensitivity or resistance, (3) altered +1 or -1 ribosomal frameshifting efficiencies, and (4) altered maintenance of the killer phenotype. Although all the mutant alleles were isolated as dominant +1 frameshift suppressors, the effects of these mutations on the cell are quite different when present as the only form of EF-1alpha. Allele-specific effects are observed with regard to their ability to alter the efficiency of programmed +1 frameshifting as opposed to programmed -1 ribosomal frameshifting. The significantly altered efficiency of -1 frameshifting in strains containing the TEF2-4 and TEF2-9 mutant alleles further correlates with a reduced ability to maintain the killer phenotype and the M1 satellite virus of L-A, an in vivo assay of translational fidelity. In light of the proposed models regarding the different A- and P-site occupancy states required for +1 or -1 ribosomal frameshifting, these results aid analysis of interactions between EF-1alpha and the translational apparatus.