Effects of D-amino acids on sleep in Drosophila
Effects of D-amino acids on sleep in Drosophila
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DOI：
10.1016/j.bbrc.2021.11.107
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发表时间：
2021-12-15
影响因子：
3.1
通讯作者：
Kume, Kazuhiko
中科院分区：
文献类型：
--
作者：
Nakagawa, Hiroyuki;Nakane, Shin;Kume, Kazuhiko
睡眠和新陈代谢密切相关，已知糖和氨基酸等营养元素对睡眠的调节方式不同。本文研究了饲料中添加D-氨基酸对果蝇睡眠的影响。在所检测的19种氨基酸中，D-丝氨酸（Ser）和D-谷氨酰胺（Gln）均能显著增加睡眠量，其中D-丝氨酸的作用最大。这种效应与其浓度成正比，并且在0.5%（约50 mM）以上显著。D-Ser可与NMDA型谷氨酸受体（NMDA type glutamate receptor，NMDAR）的NR 1亚基结合并激活，但对NR 1亚基亚基亚此外，分解代谢D-氨基酸的D-氨基酸氧化酶（Daao 1）的亚型突变体，已知其破坏会增加大脑中的D-丝氨酸，显示睡眠增加。这些结果共同表明，D-Ser激活大脑中的NMDAR，从而增加睡眠，并且D-Ser在生理上调节睡眠。(c)2021爱思唯尔公司All rights reserved.
Sleep and metabolism are closely related and nutritional elements such as sugars and amino acids are known to regulate sleep differently. Here we comprehensively investigated the effects of D-amino acids fed in the diet on the sleep of Drosophila melanogaster. Among 19 amino acids examined, both D-serine (Ser) and D-glutamine (Gln) induced a significant increase in sleep amount and the effect of D-Ser was the largest at the same concentration of 1% of the food. The effects were proportional to its concentration and significant above 0.5% (about 50 mM). D-Ser is known to bind NR1 subunit of NMDA type glutamate receptor (NMDAR) and activate it. D-Ser did not increase the sleep of the NR1 hypomorphic mutant flies indicating its effects on sleep is mediated by NMDAR. In addition, hypomorphic mutants of D-amino acid oxidase (Daao1), which catabolizes D-amino acids and its disruption is known to increase D-Ser in the brain, showed increase in sleep. These results altogether suggested that D-Ser activated NMDAR in the brain thus increase sleep, and that D-Ser work physiologically to regulate sleep. (c) 2021 Elsevier Inc. All rights reserved.