Characterization and metabolism of ovine foetal lipids.
Characterization and metabolism of ovine foetal lipids.
复制标题
绵羊胎儿脂质的表征和代谢。
作者：
T. W. Scott;B. Setchell;J. Bassett
1.140日龄绵羊胎儿肝脏、肾脏和脑中的总磷脂含量仅为可比母体组织的一半。2.所有受检胎儿组织中，磷脂酰胆碱是主要磷脂。胎儿和母体组织在个体磷脂方面最显著的差异是心脏；胎儿心脏含有更多的乙醇胺血浆原而不是胆碱血浆原，而在成人组织中这两种物质的浓度正好相反。胎儿脑鞘磷脂含量仅为母体脑组织的六分之一。3.从胎儿组织中提取的磷脂以油酸(18：1)为主，脑组织中棕榈酸(16：0)含量稍高。在成体组织的磷脂中，不饱和脂肪酸的比例较高(亚油酸为18：2，亚麻酸为18：3)，油酸的比例相对较低(18：1)。胎儿和母体组织中性脂肪组分中脂肪酸的分布非常相似，油酸(18：1)一般是主要成分。4.(14)将[U-(14)C]-葡萄糖和[U-(14)C]果糖在胎儿宫内循环中注入心、肝、肾、脑和脂肪组织的中性脂和磷脂中。5.磷脂分析表明，肝脏中磷脂酸的比活性高于其他组织。在心脏组织中，磷脂酰乙醇胺的比活性低于磷脂酰胆碱，但在其他组织中的比活性大致相同。脑组织中磷脂酰肌醇和磷脂酸的比活性非常相似，均高于其他组分。在肝脏和棕色脂肪中，磷脂酰丝氨酸的比活性最高。6.来自[(14)C]葡萄糖和[(14)C]果糖的(14)C在胎儿中性脂中的掺入模式相似。甘油三酯在脑中的放射性占大部分，而甘油三酯在心、肝、肾和脂肪中的标记较多。
1. Total phospholipid concentrations in liver, kidney and brain of the 140-day ovine foetus were only half of those in comparable maternal tissues. 2. Phosphatidylcholine was the predominant phospholipid in all foetal tissues examined. The most striking difference between foetal and maternal tissues in individual phospholipids was in the heart; foetal heart contained more ethanolamine plasmalogen than choline plasmalogen, whereas in adult tissue the concentration of these was reversed. Sphingomyelin content of foetal brain was only one-sixth of that of maternal brain tissue. 3. Oleic acid (18:1) was the predominant acid in the phospholipid extracted from foetal tissues, except in brain where palmitic acid (16:0) was slightly higher. In phospholipids from adult tissues there was a higher proportion of unsaturated fatty acids (linoleic acid, 18:2, and linolenic acid, 18:3) and a correspondingly lower proportion of oleic acid (18:1). The distribution of fatty acids in the neutral lipid fraction of foetal and maternal tissues was very similar; oleic acid (18:1) was generally the principal component. 4. (14)C derived from [U-(14)C]-glucose and [U-(14)C]fructose infused into the foetal circulation in utero was incorporated into the neutral lipids and phospholipids of heart, liver, kidney, brain and adipose tissue. 5. Phospholipid analysis revealed that the specific activity of phosphatidic acid was higher in liver than in other tissues. The specific activity of phosphatidylethanolamine was less than that of phosphatidylcholine in heart, but in other tissues they were about the same. The specific activities of phosphatidylinositol and phosphatidic acid in brain were very similar and were higher than the other components. The specific activity of phosphatidylserine was highest in liver and brown fat. 6. The pattern of incorporation of (14)C derived from [(14)C]glucose and [(14)C]fructose into foetal neutral lipids was similar. Diglyceride accounted for most of the radioactivity in brain, whereas triglyceride had more label in heart, liver, kidney and fat.