Computerized video analysis of social interactions in mice
Computerized video analysis of social interactions in mice
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DOI：
10.1038/nmeth.1924
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发表时间：
2012-04-01
期刊：
影响因子：
48
通讯作者：
Olivo-Marin, Jean-Christophe
中科院分区：
文献类型：
--
作者：
de Chaumont, Fabrice;Coura, Renata Dos-Santos;Olivo-Marin, Jean-Christophe
对小鼠社交互动的研究被用作正常和病理认知和情绪过程的模型。但从交互小鼠的视频中提取全面的行为信息仍然是一个挑战。我们描述了一种计算机化的方法和软件，MiceProfiler，它使用几何基元来建模和跟踪两只老鼠，而不需要任何特定的标记。该程序监控全面的行为状态及其时间演变，从而识别触发社交接触的关键要素。使用 MiceProfiler，我们研究了神经元烟碱受体在建立社交互动和危险姿势中的作用。我们发现，与 C57BL/6J 小鼠相比，缺乏神经元烟碱受体（Chrnb2(-/-)，此处称为 beta 2(-/-)）的小鼠与同种动物的社交互动的持续时间和类型随着时间的推移而发生变化，并确定了一种新型协调姿势，称为背对背姿势，我们很少在 beta(2-/-) 小鼠中观察到这种姿势。
T he study of social interactions in mice is used as a model for normal and pathological cognitive and emotional processes. But extracting comprehensive behavioral information from videos of interacting mice is still a challenge. We describe a computerized method and software, MiceProfiler, that uses geometrical primitives to model and track two mice without requiring any specific tagging. The program monitors a comprehensive repertoire of behavioral states and their temporal evolution, allowing the identification of key elements that trigger social contact. Using MiceProfiler we studied the role of neuronal nicotinic receptors in the establishment of social interactions and risk-prone postures. We found that the duration and type of social interactions with a conspecific evolves differently over time in mice lacking neuronal nicotinic receptors (Chrnb2(-/-), here called beta 2(-/-)), compared to C57BL/6J mice, and identified a new type of coordinated posture, called back-to-back posture, that we rarely observed in beta(2-/-) mice.