High-fidelity Fourier transform infrared spectroscopic imaging of primate brain tissue
High-fidelity Fourier transform infrared spectroscopic imaging of primate brain tissue
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DOI：
10.1366/0003702963906618
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发表时间：
1996-02-01
影响因子：
3.5
通讯作者：
Levin, IW
中科院分区：
文献类型：
--
作者：
Lewis, EN;Gorbach, AM;Levin, IW
我们展示了一种新的中红外和近红外成像方法，该方法非常适合微观应用。该方法采用锑化铟 (InSb) 焦平面阵列探测器和市售的步进扫描傅里叶变换红外光谱仪 (FTIR)。使用 KBr 或 CaF2 分束器，可以利用探测器上的所有可用像素快速获取 1 至 5.5 μ m (10,000-1818 cm(-1)) 的图像。通过改变干涉仪扫描期间采集的图像数量，可以轻松改变每个图像的光谱分辨率。我们应用这种技术在猴脑组织切片中无创地生成图像对比度，并将这些数据与特定的脂质和蛋白质组分相关联。此外，我们描述了几种计算方法来突出样本内成分的空间分布。
We demonstrate a new mid-infrared and near-infrared imaging approach which is ideally suited to microscopic applications. The method employs an indium antimonide (InSb) focal-plane array detector and a commercially available step-scan Fourier transform infrared spectrometer (FTIR). With either a KBr or a CaF2 beamsplitter, images from 1 to 5.5 mu m (10,000-1818 cm(-1)) can be rapidly acquired with the use of all the available pixels on the detector. The spectral resolution for each image is easily varied by changing the number of acquired images during the interferometer scan. We apply this technique to noninvasively generate image contrast in sections of monkey brain tissue and to relate these data to specific lipid and protein fractions. In addition, we describe several computational methods to highlight the spatial distributions of components within a sample.