Single-copy DNA distance between two congeneric sea urchin species exhibiting radically different modes of development.
Single-copy DNA distance between two congeneric sea urchin species exhibiting radically different modes of development.
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两个同属海胆物种之间的单拷贝 DNA 距离表现出截然不同的发育模式。
DOI：
10.1093/oxfordjournals.molbev.a040610
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发表时间：
1990
影响因子：
10.7
通讯作者：
Raff,RA
中科院分区：
文献类型：
--
作者：
Smith,MJ;Boom,JD;Raff,RA
我们已经调查了两个据称同源物种的海胆，从根本上不同的早期发展的核基因组之间的差异。具瘤杀螟Heliocidaris tuberculata的发育方式为典型的pluteus幼虫，而H.红带单胞菌直接从体积是红带单胞菌100倍的卵中发育而成。具瘤卵重组动力学分析表明，两种物种的基因组DNA的动力学成分基本相同。没有单一的重复成分解释H. erythrogramma和H. tuberculata基因组。这些物种之间的示踪剂标记的单拷贝DNA片段的相互杂交表明，大约50%的单拷贝DNA是足够相似的，以形成杂交在标准杂交标准。杂交的单拷贝DNA序列的热变性谱产生13.8 ℃-16.5 ℃的中值（T50 H）值。这一结果表明，分歧时间为10-13万年，这是可比的其他属的同源海胆物种之间的分歧时间，没有显着不同的发展。早期发育过程中的根本差异可以在密切相关的形式之间迅速演变。
We have investigated the differences between nuclear genomes of two purportedly congeneric species of sea urchin that differ radically in early development. Heliocidaris tuberculata develops by means of a typical pluteus larva, whereas H. erythrogramma develops directly from an egg that is 100-fold the volume of the H. tuberculata egg. Reassociation kinetic analysis shows that the kinetic components of the genomic DNA from the two species are essentially the same. No single repeat component explains the 30% difference between the H. erythrogramma and H. tuberculata genomes. Reciprocal hybridization of tracer-labeled single-copy DNA fractions between these species indicates that approximately 50% of the single-copy DNA is sufficiently similar to form hybrids at standard hybridization criterion. Thermal denaturation profiles of the hybridized single-copy DNA sequence yields median (T50H) values of 13.8 degrees-16.5 degrees C. This result suggests a divergence time of 10-13 Mya, which is comparable to divergence times between congeneric sea urchin species in other genera that do not differ significantly in development. Radical differences in early developmental processes can evolve rapidly between closely related forms.