Polyethylene particles stimulate expression of ITAM-related molecules in peri-implant tissues and when stimulating osteoclastogenesis in vitro
Polyethylene particles stimulate expression of ITAM-related molecules in peri-implant tissues and when stimulating osteoclastogenesis in vitro
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DOI：
10.1016/j.actbio.2012.04.037
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发表时间：
2012-08-01
影响因子：
9.7
通讯作者：
Haynes, D. R.
中科院分区：
文献类型：
--
作者：
Alias, E.;Dharmapatni, A. S. S. K.;Haynes, D. R.
磨损颗粒诱导的骨科假体松动与破骨细胞活性升高相关。基于免疫受体酪氨酸的激活基序（ITAM）相关分子OSCAR、FcR γ、TREM 2和DAP 12对于破骨细胞形成是重要的。本研究的目的是通过研究这些分子在翻修含聚乙烯（PE）磨损颗粒的关节置换组件时获得的笔式植入物组织中的表达，以及在存在PE颗粒的体外形成的破骨细胞中的表达，确定这些分子是否参与笔式植入物松动。结果表明，在翻修组织中ITAM相关分子的蛋白水平有显著的统计学显著性增加。修复组织中OSCAR、FcR γ、TREM 2和DAP 12 mRNA水平也升高。体外PE颗粒在50 ng ml（-1）受体激活剂NF κ B（RANKL）存在下刺激破骨细胞再吸收，并在破骨细胞形成过程中显著升高OSCAR、FcR γ、TREM 2和DAP 12的表达。这些发现表明，ITAM信号分子及其共受体在与种植体PE磨损相关的致病性骨丢失中发挥作用。(c)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Wear particle-induced orthopaedic prosthesis loosening is associated with elevated osteoclast activity. The immunoreceptor tyrosine-based activation motif (ITAM)-related molecules OSCAR, FcR gamma, TREM2 and DAP12 are important for osteoclast formation. The aim of this study was to determine if these molecules are involved in pen-implant loosening by investigating their expression in pen-implant tissues obtained at revision of joint replacement components containing polyethylene (PE) wear particles, and in osteoclasts formed in vitro in the presence of PE particles. The results showed that there was a marked and statistically significant increase in protein levels of the ITAM-related molecules in the revision tissues. The levels of OSCAR, FcR gamma, TREM2 and DAP12 mRNA in the revision tissues were also increased. In vitro PE particles stimulated osteoclast resorption in the presence of 50 ng ml(-1) receptor activator NF kappa B (RANKL) and significantly elevated the expression of OSCAR, FcR gamma, TREM2 and DAP12 during osteoclast formation. These findings suggest that the ITAM signalling molecules and their co-receptors have a role in pathogenic bone loss associated with implant PE wear. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.