Biohydrogen production from a novel alkalophilic isolate Clostridium sp. IODB-O3.
Biohydrogen production from a novel alkalophilic isolate Clostridium sp. IODB-O3.
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从新型嗜碱梭状芽胞杆菌分离物中生产生物氢。
DOI：
10.1016/j.biortech.2014.10.110
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发表时间：
2015
影响因子：
11.4
通讯作者：
D. Tuli
中科院分区：
文献类型：
--
作者：
A. Patel;Arundhati Debroy;Sandeep Sharma;Reetu Saini;A. Mathur;Ravi P. Gupta;D. Tuli
从污泥中分离到一株产氢细菌IODB-O3，经鉴定为梭菌属（Clostridiumsp.）16 S rDNA基因分析。本研究利用低成本的农业废弃物开发生物制氢工艺。在37 °C和pH 8.5下产生最大H2。预处理后的小麦秸秆酶解液和预处理后的小麦秸秆酶解液的最大产氢量分别为2.54 ± 0. 2 mol-H2/mol还原糖和2.61 ± 0. 1 mol-H2/mol还原糖。WSPH和WSEH的累积产氢量（ml/L）分别为3680 ± 105和3270 ± 100，产氢速率（ml/L/h）分别为153 ± 5和136 ± 5，比产氢量（ml/g/h）分别为511 ± 5和681 ± 10。通过蒸汽爆破的生物质预处理产生了大量的WSPH，由于高效C5发酵微生物的不可用，WSPH仍未用于生物乙醇生产。本研究表明，Clostridium sp. IODB-O3能够有效地利用WSPH进行生物制氢。这将导致减少对整个纤维素乙醇工艺的经济约束，并且还建立可持续的生物氢生产工艺。
Hydrogen producing bacteria IODB-O3 was isolated from sludge and identified asClostridiumsp. by 16S rDNA gene analysis. In this study, biohydrogen production process was developed using low-cost agro-waste. Maximum H2was produced at 37 °C and pH 8.5. Maximum H2yield was obtained 2.54 ± 0.2 mol-H2/mol-reducing sugar from wheat straw pre-hydrolysate (WSPH) and 2.61 ± 0.1 mol-H2/mol-reducing sugar from pre-treated wheat straw enzymatic-hydrolysate (WSEH). The cumulative H2production (ml/L), 3680 ± 105 and 3270 ± 100, H2production rate (ml/L/h), 153 ± 5 and 136 ± 5, and specific H2production (ml/g/h), 511 ± 5 and 681 ± 10 with WSPH and WSEH were obtained, respectively. Biomass pre-treatment via steam-explosion generates ample amount of WSPH which remains unutilized for bioethanol production due to non-availability of efficient C5-fermenting microorganisms. This study shows thatClostridiumsp. IODB-O3 is capable of utilizing WSPH efficiently for biohydrogen production. This would lead to reduced economic constrain on the overall cellulosic ethanol process and also establish a sustainable biohydrogen production process.