Susceptibility testing with the manual mycobacteria growth indicator tube (MGIT) and the MGIT 960 system provides rapid and reliable verification of multidrug-resistant tuberculosis
Susceptibility testing with the manual mycobacteria growth indicator tube (MGIT) and the MGIT 960 system provides rapid and reliable verification of multidrug-resistant tuberculosis
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DOI：
10.1128/jcm.41.3.1235-1239.2003
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发表时间：
2003-03-01
影响因子：
9.4
通讯作者：
Katila, ML
中科院分区：
文献类型：
--
作者：
Ådjers-Koskela, K;Katila, ML
本研究的目的是比较手动分枝杆菌生长指示管（MGIT）方法和BACTEC MGIT 960系统与BACTEC 460方法对结核分枝杆菌的药敏试验。该评价基于36 M的试验。对异烟肼（INH）、利福平（RMP）、乙胺丁醇（EMB）和链霉素（SM）具有不同敏感性的结核菌株。此外，对EMB检测中产生不一致结果的5种菌株进行了异源耐药性分析。对于INH，MGIT 960和手动MGIT系统获得的药敏试验结果分别在94%和97%的情况下与BACTEC 460结果一致。对RMP的敏感性测定结果一致。对于SM，MGIT 960和手动MGIT系统获得的结果分别有78%和72%与BACTEC 460结果一致。与此相反，不到80%的结果，EMB的敏感性得到的两个MGIT方法同意与BACTEC 460的结果。所有五个菌株分析EMB异源抗性被发现由耐药和敏感亚群。MGIT 960系统的平均周转时间为6.4天，手动MGIT系统为6.5天，BACTEC 460方法为8.7天。两种MGIT方法均可被视为BACTEC 460方法的准确和快速替代方法，用于检测INH和RMP耐药菌株。然而，需要更多的研究来解决与EMB和SM的敏感性测试相关的问题。
The objective of the study was to compare the manual Mycobacteria Growth Indicator Tube (MGIT) method and the BACTEC MGIT 960 system to the BACTEC 460 method for susceptibility testing of Mycobacterium tuberculosis. The evaluation was based on testing of 36 M. tuberculosis strains with various susceptibilities to isoniazid (INH), rifampin (RMP), ethambutol (EMB), and streptomycin (SM). In addition, five of the strains generating discrepant results in testing for EMB were analyzed for heteroresistance. For INH, the susceptibility test results obtained by the MGIT 960 and the manual MGIT systems agreed with the BACTEC 460 results in 94 and 97% of the cases, respectively. The results of susceptibility to RMP were all in agreement. For SM, 78 and 72% of the results obtained by the MGIT 960 and the manual MGIT systems, respectively, agreed with the BACTEC 460 results. In contrast, less than 80% of the results for susceptibility to EMB obtained by the two MGIT methods agreed with the BACTEC 460 results. All five strains analyzed for EMB heteroresistance were found to consist of resistant and susceptible subpopulations. The average turnaround times were 6.4 days for the MGIT 960 system, 6.5 for the manual MGIT system, and 8.7 days for the BACTEC 460 method. Both MGIT methods can be regarded as accurate and rapid alternatives to the BACTEC 460 method for detection of strains resistant to INH and RMP. However, more studies are needed for solving the problems associated with susceptibility testing to EMB and SM.