A new method for the direct determination of serum cholesterol.
A new method for the direct determination of serum cholesterol.
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直接测定血清胆固醇的新方法
DOI：
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发表时间：
1953
期刊：
影响因子：
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通讯作者：
A. Boyle
中科院分区：
文献类型：
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作者：
A. Zlatkis;A. Zlatkis;B. Zak;B. Zak;A. Boyle;A. Boyle
本文介绍了一种直接定量测定血清总胆固醇的方法。该程序包括将含有硫酸、乙酸和氯化铁的试剂加入到0.1 ml的血清样本。所实现的灵敏度是当前程序的几倍，并且单次测定所需的时间为几分钟。证明了精密度和重现性以及通过400至700 mμ的光谱范围产生的紫色的吸收特性。最后，该方法进行了比较，其中总胆固醇是由Kingsley-Schaffert和Schoenheimer-Sperry程序确定的样品。
Abstract A direct method for the quantitative determination of total serum cholesterol is described. The procedure consists of adding a reagent containing sulfuric acid, acetic acid, and ferric chloride to a 0.1 ml. sample of serum. The sensitivity achieved is several times that of current procedures and the time required for a single determination is in the order of minutes. Precision and reproducibility are demonstrated as well as the absorption characteristics of the purple color produced through the spectral range of 400 to 700 mμ. Finally, the method is compared with samples in which total cholesterol is determined by the Kingsley-Schaffert and Schoenheimer-Sperry procedures.