Widespread expression of rat collapsin response‐mediated protein 4 in the telencephalon and other areas of the adult rat central nervous system
Widespread expression of rat collapsin response‐mediated protein 4 in the telencephalon and other areas of the adult rat central nervous system
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DOI：
10.1002/1096-9861(20000904)424:4
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发表时间：
2000-09
影响因子：
2.5
通讯作者：
J. Nácher;D. Rosell;B. McEwen
中科院分区：
文献类型：
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作者：
J. Nácher;D. Rosell;B. McEwen
大鼠坍缩反应介导蛋白4 （rCRMP - 4）是参与轴突生长的蛋白家族的一员。它在有丝分裂后的神经元中短暂存在，例如在成人海马中产生的神经元。作者使用免疫细胞化学方法来研究大鼠中枢神经系统（CNS）保留出生后神经发生的区域是否表达这种蛋白。他们发现齿状颗粒层、脑室下区、嗅球和吻侧迁移流中新近生成的细胞具有明显的rCRMP‐4免疫反应性，这四个区域在成年期发生神经元的产生或迁移。然而，rCRMP‐4的免疫反应性也在大鼠大脑的许多其他区域表达，这些区域没有成年神经发生或神经元迁移的记录，例如在嗅肾小球和大脑皮层的神经元中。在下丘脑，rCRMP‐4强烈标记的神经元分布在视上核、室旁核、室周核以及正中隆起核和弓状核。rCRMP‐4的免疫反应性也存在于核间核、中缝、上丘和分散颗粒小脑的某些神经元中。已知这些区域中的许多在成年期表现出轴突生长和/或突触重排，并共同表达神经细胞粘附分子的多唾液化形式。因此，本研究结果表明rCRMP‐4在中枢神经系统中的表达与正在迁移或正在进行轴突生长的细胞有关。然而，小的rCRMP - 4免疫反应细胞在整个大脑中可见。这些细胞不表达神经元、星形胶质细胞或小胶质细胞标记物，尽管其中一些细胞对撕裂抗体也有免疫反应，表明它们具有少突胶质细胞谱系。[j]中华神经科杂志，2004,24(4):628 - 639。©2000 Wiley‐Liss, Inc。
The rat collapsin response‐mediated protein 4 (rCRMP‐4) is a member of a family of proteins that are involved in axonal growth. It is found transiently in postmitotic neurons, such as those that are generated in the adult hippocampus. The authors used immunocytochemistry to investigate whether areas of the rat central nervous system (CNS) that retain postnatal neurogenesis express this protein. They found pronounced rCRMP‐4 immunoreactivity in recently generated cells in the dentate granular layer, the subventricular zone, the olfactory bulbs, and the rostral migratory stream, four areas in which the production or migration of neurons occurs in adulthood. However, rCRMP‐4 immunoreactivity also is expressed in many other regions of the rat brain in which there is no record of adult neurogenesis or neuronal migration, e.g., in the olfactory glomeruli and in neurons of the cerebral cortex. In the hypothalamus, intensely rCRMP‐4‐labeled neurons populated the supraoptic, paraventricular, and periventricular nuclei as well as the median eminence and the arcuate nucleus. Immunoreactivity for rCRMP‐4 also was present in certain neurons of the interpeduncular nucleus, median raphe, superior colliculus, and scattered granule cerebellar neurons. Many of these regions are known to display axonal outgrowth and/or synaptic rearrangement in adulthood and to coexpress the polysialylated form of the neural cell adhesion molecule. Thus, the results of this study suggest that rCRMP‐4 expression in the CNS is associated with cells that are migrating or are undergoing axonal growth. Nevertheless, small, rCRMP‐4‐immunoreactive cells were seen throughout the brain. These cells did not express neuronal, astroglial, or microglial markers, although some of them also were immunoreactive for rip antibody, suggesting an oligodendroglial lineage. J. Comp. Neurol. 424:628–639, 2000. © 2000 Wiley‐Liss, Inc.