DNA rearrangements in human follicular lymphoma can involve the 5' or the 3' region of the bcl-2 gene.
DNA rearrangements in human follicular lymphoma can involve the 5' or the 3' region of the bcl-2 gene.
复制标题
人滤泡性淋巴瘤中的 DNA 重排可能涉及 bcl-2 基因的 5 或 3 区域。
DOI：
10.1073/pnas.84.5.1329
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发表时间：
1987
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Tsujimoto,Y;Bashir,MM;Givol,I;Cossman,J;Jaffe,E;Croce,CM
在大多数人滤泡性淋巴瘤中，染色体易位t(14；18)发生在18号染色体上的两个断裂点聚集区，主要的易位在bcl2基因的3‘非翻译区，次要的易位在该基因的3’非翻译区。使用bcl2基因第一外显子的探针对一组滤泡性淋巴瘤DNA进行分析表明，涉及的bcl2基因也可能发生DNA重排5‘。在这个病例中，发现了IgH基因座和bcl2基因，其顺序为3‘C伽马S伽马/亩JH 5’：：5‘BCL-2 3’(其中C=常量，S=开关，JH=重链基因的连接段)，提示在易位过程中也发生了倒位。然而，到目前为止，所有研究的滤泡性淋巴瘤病例中，bcl2基因的编码区都保持完好。
In most human follicular lymphomas, the chromosome translocation t(14;18) occurs within two breakpoint clustering regions on chromosome 18, the major one at the 3' untranslated region of the bcl-2 gene and the minor one at 3' of the gene. Analysis of a panel of follicular lymphoma DNAs using probes for the first exon of the bcl-2 gene indicates that DNA rearrangements may also occur 5' to the involved bcl-2 gene. In this case the IgH locus and the bcl-2 gene are found in the order 3' C gamma S gamma/mu JH 5'::5' bcl-2 3' (where C = constant, S = switch, and JH = joining segment of the heavy chain locus), suggesting that an inversion also occurred during the translocation process. The coding regions of the bcl-2 gene, however, are left intact in all cases of follicular lymphoma studied to date.