An allele of the 1-aminocyclopropane-1-carboxylate synthase gene (Md-ACS1) accounts for the low level of ethylene production in climacteric fruits of some apple cultivars
An allele of the 1-aminocyclopropane-1-carboxylate synthase gene (Md-ACS1) accounts for the low level of ethylene production in climacteric fruits of some apple cultivars
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DOI：
10.1007/s001220051539
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发表时间：
2000-10-01
影响因子：
5.4
通讯作者：
Niizeki, M
中科院分区：
文献类型：
--
作者：
Harada, T;Sunako, T;Niizeki, M
苹果成熟特异性1-氨基环丙烷-1-羧酸（ACC）合酶基因（Md-ACS 1 -1）的等位基因具有5 ′侧翼区，该侧翼区具有插入的逆转录子样序列。苹果物种可以分为三个组，是杂合或纯合的ACS 1 -1和ACS 1 -2等位基因。测定了35个不同基因型苹果跃变型果实内乙烯浓度（IEC）。11个ACS 1 -2纯合品种表现出比ACS 1 -1纯合或杂合品种低得多的IEC。此外，F-1 ACS 1 -2纯合后代来自杂合品种之间的杂交具有非常低的IEC的果实。这些结果与先前的数据雅阁，表明在杂合栽培品种中不存在来自ACS 1 -2的转录。由于在几个ACS 1 -2纯合品种的跃变型果实中观察到低水平的ACS 1 mRNA，我们得出结论，在一些品种中的乙烯产生的低水平是由突变的等位基因的ACS 1，这是负责在成熟过程中的乙烯产生的主要基因。
An allele of the apple ripening-specific 1-aminocyclopropane-1-carboxylate (ACC) synthase gene (Md-ACS1-1) has a 5'-flanking region possessing an inserted retroposon-like sequence. Apple species can be classified into three groups that are heterozygous or homozygous for the ACS1-1 and ACS1-2 alleles. We measured the internal ethylene concentration (IEC) in climacteric fruit of 35 apple cultivars with respect to genotype. Eleven ACS1-2 homozygous cultivars exhibited much lower IECs than cultivars homozygous or heterozygous for ACS1-1. Furthermore, F-1 ACS1-2 homozygous progeny derived from crosses between heterozygous cultivars had fruit with a very low IEC. These results are in accord with previous data indicating the absence of transcription from ACS1-2 in a heterozygous cultivar. Since the low level of ACS1 mRNA in climacteric fruit was observed in several ACS1-2 homozygous cultivars, we conclude that the low level of ethylene production in some cultivars is caused by the mutated allele of ACS1, which is the main gene responsible for ethylene production during ripening.