Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridization
Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridization
复制标题
通过斑点印迹和原位杂交对烟草不对称体细胞杂种进行染色体分析
DOI：
10.1007/bf00283029
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
G. Bates
中科院分区：
文献类型：
--
作者：
W. Piastuch;G. Bates
从白花烟草（Nicotiana plumbaginifolia）中克隆了一个种特异的、分散的重复DNA序列，并利用斑点杂交和原位杂交技术分析了白花烟草（Nicotiana plumbaginifolia）的体细胞杂种。烟草卡那霉素抗性N.白花丹用克隆的种特异性重复DNA作探针进行斑点杂交，结果表明，有些杂种仅含1%~ 5%的N。白花丹DNA含量为15%~ 25%。探针与染色体的原位杂交表明，极不对称的杂交体保留了一个单一的N。斑点杂交值高的杂种，其染色体N值为8 ~ 12。白花丹染色体和染色体片段。原位杂交还揭示了N.白花丹和N.在8个杂种中，有3个杂种的染色体为短轴。用5S基因探针进行的RFLP分析表明，N.在大多数杂种检查，包括那些只保留一个singleN。白花丹染色体
A species-specific, dispersed repetitive DNA sequence was cloned fromNicotiana plumbaginifoliaand used in dot blots and in situ hybridizations to analyze asymmetric somatic hybrids ofN. tabacum(+)kanamycin-resistantN. plumbaginifolia. Dot blot hybridization data, using the cloned, species-specific repetitive DNA as a probe, showed that some of the hybrids contain only 1%–5%N. plumbaginifoliaDNA, whereas others contain 15%–25%. In situ hybridization of the probe to chromosome spreads showed that the extremely asymmetric hybrids retain a singleN. plumbaginifoliachromosome; the hybrids with higher dot blot values were found to have 8 to 12N. plumbaginifoliachromosomes and chromosome fragments. In situ hybridization also revealed translocations betweenN. plumbaginifoliaandN. tabacumchromosomes in 3 of 8 hybrids studied. RFLP analysis using a 5S gene probe showed the presence ofN. plumbaginifolia-specific 5S banding patterns in most hybrids examined, including those that retain only a singleN. plumbaginifoliachromosome.
DOI：
--
发表时间：
2002
期刊：
Plant Biotechnology 19
影响因子：
--
作者：
Chen;L.Z.
通讯作者：
L.Z.