Propagation of Chrysanthemum in Vitro. I. Multiple Plantlets from Shoot Tips and the Establishment of Tissue Cultures1,2
Propagation of Chrysanthemum in Vitro. I. Multiple Plantlets from Shoot Tips and the Establishment of Tissue Cultures1,2
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DOI：
10.21273/jashs.99.2.128
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发表时间：
1974-03
影响因子：
1.9
通讯作者：
E. Earle;R. Langhans
中科院分区：
文献类型：
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作者：
E. Earle;R. Langhans
将菊花“Giant #4 Indianapolis白色”的小（0.5 mm高）茎尖生长在含有各种水平激动素、NAA和GA 3的Murashige-Skoog培养基上。根、单个或多个芽、小植株和易碎、坚硬或叶状愈伤组织的形成取决于所使用的激素水平。在含激动素2.0 mg/l和NAA 0.02 mg/l的培养基上，可产生丛生芽和绿色叶片愈伤组织。叶片状愈伤组织适合继代培养和植株重组。使多个芽生根并生长成正常植物或用于开始形成更多多个芽的新培养物。该技术为菊花的贮藏和繁殖，特别是脱毒植株的检测和快速繁殖提供了技术依据。
Small (0.5 mm high) shoot tips of Chrysanthemum morifolium ‘Giant #4 Indianapolis White’ were grown on Murashige-Skoog medium containing various levels of kinetin, NAA, and GA3. Formation of roots, single or multiple shoots, plantlets and friable, hard or leafy callus depended on the hormone levels used. Multiple shoots and green leafy callus were produced on medium containing 2.0 mg/l kinetin and 0.02 mg/l NAA. The leafy callus was suitable for subculture and subsequent reorganization of plantlets. Multiple shoots were rooted and grown into normal plants or were used to start new cultures which formed more multiple shoots. This technique will be useful for storage and propagation of Chrysanthemum and especially for detection and rapid multiplication of virus-free plants.