Influence of Mpv17 on Hair-Cell Mitochondrial Homeostasis, Synapse Integrity, and Vulnerability to Damage in the Zebrafish Lateral Line.
Influence of Mpv17 on Hair-Cell Mitochondrial Homeostasis, Synapse Integrity, and Vulnerability to Damage in the Zebrafish Lateral Line.
复制标题
DOI：
10.3389/fncel.2021.693375
复制
发表时间：
2021
影响因子：
5.3
通讯作者：
Sheets L
中科院分区：
文献类型：
--
作者：
Holmgren M;Sheets L
Noise exposure is particularly stressful to hair-cell mitochondria, which must produce enough energy to meet high metabolic demands as well as regulate local intracellular Ca2+ concentrations. Mitochondrial Inner Membrane Protein 17 (Mpv17) functions as a non-selective cation channel and plays a role in maintaining mitochondrial homeostasis. In zebrafish, hair cells in mpv17a9/a9 mutants displayed elevated levels of reactive oxygen species (ROS), elevated mitochondrial calcium, hyperpolarized transmembrane potential, and greater vulnerability to neomycin, indicating impaired mitochondrial function. Using a strong water current to overstimulate hair cells in the zebrafish lateral line, we observed mpv17a9/a9 mutant hair cells were more vulnerable to morphological disruption than wild type (WT) siblings simultaneously exposed to the same stimulus. To determine the role of mitochondrial homeostasis on hair-cell synapse integrity, we surveyed synapse number in mpv17a9/a9 mutants and WT siblings as well as the sizes of presynaptic dense bodies (ribbons) and postsynaptic densities immediately following stimulus exposure. We observed mechanically injured mpv17a9/a9 neuromasts were not more vulnerable to synapse loss; they lost a similar number of synapses per hair cell relative to WT. Additionally, we quantified the size of hair cell pre- and postsynaptic structures following stimulation and observed significantly enlarged WT postsynaptic densities, yet relatively little change in the size of mpv17a9/a9 postsynaptic densities following stimulation. These results suggest chronically impaired hair-cell mitochondrial activity influences postsynaptic size under homeostatic conditions but does not exacerbate synapse loss following mechanical injury.
登录
查看更多内容
影响因子：
4.5
作者：
Behra M;Bradsher J;Sougrat R;Gallardo V;Allende ML;Burgess SM
通讯作者：
Burgess SM
影响因子：
2.7
作者：
D'Agati G;Beltre R;Sessa A;Burger A;Zhou Y;Mosimann C;White RM
通讯作者：
White RM
影响因子：
3.4
作者：
Ivannikov, Maxim V.;Macleod, Gregory T.
通讯作者：
Macleod, Gregory T.
影响因子：
6
作者：
Binder, CJ;Weiher, H;Kerjaschki, D
通讯作者：
Kerjaschki, D
影响因子：
4.8
作者：
Antonenkov, Vasily D.;Isomursu, Antti;Hiltunen, J. Kalervo
通讯作者：
Hiltunen, J. Kalervo