Effect of All-Trans Retinoic Acid on the Pancreas of Streptozotocin-Induced Diabetic Rat
Effect of All-Trans Retinoic Acid on the Pancreas of Streptozotocin-Induced Diabetic Rat
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DOI：
10.1002/ar.23307
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发表时间：
2016-03-01
影响因子：
2
通讯作者：
El-Metwally, Tarek H.
中科院分区：
文献类型：
--
作者：
Eltony, Sohair A.;Elmottaleb, Nashwa A.;El-Metwally, Tarek H.
全反式维甲酸（atRA）对内分泌胰腺的发育具有指导意义，是β细胞诱导方案的组成部分。我们发现，atRA诱导葡萄糖反应性内分泌转分化的多形性胰腺导管腺癌细胞在体外。本研究旨在探讨全反式维甲酸对糖尿病大鼠胰腺组织学改变的改善作用。40只雄性Wistar大鼠随机分为3组。I组：正常溶剂对照组（N=5）。第二组：链脲佐菌素诱导的糖尿病大鼠（N=20），在0.0，1，2，4周进行随访。第三组：链脲佐菌素诱导的糖尿病大鼠（N=15），用atRA（2.5 mg/kg/d）治疗，在第1、2和4周进行随访。胰腺标本进行光镜、电镜和胰腺胰岛素mRNA表达。血液样本的葡萄糖，胰岛素和总过氧化物的水平进行了分析。atRA治疗组中，胰岛数量和面积显着增加。在胰腺腺泡和小叶间导管附近观察到强烈的胰岛素免疫反应性内分泌样细胞。有趣的是，胰岛素阳性细胞似乎来自胰腺腺泡和导管上皮。超微结构上，β细胞，腺泡和导管细胞恢复了正常的外观。胰腺胰岛素mRNA和血液指标基本正常。AtRA可改善糖尿病大鼠胰腺组织学改变和血液指标。(C)2015 Wiley Periodicals，Inc.
All-trans Retinoic acid (atRA) is instructive for the development of endocrine pancreas and is an integral component of beta-cell induction protocols. We showed that atRA induces glucose-responsive endocrine trans-differentiation of pleomorphic pancreatic ductal adenocarcinoma cells in vitro. This study aimed to detect the role of atRA in improving the histological changes of the pancreas in diabetic rats. Forty young male Wistar rats were used and divided into three groups. Group I: normal vehicle control (N=5). Group II: streptozotocin-induced diabetic rats (N=20) were followed up at 0.0, 1, 2, and 4 weeks. Group III: streptozotocininduced diabetic rats (N=15) treated with atRA (2.5 mg/kg/day), were followed up at 1, 2, and 4 weeks. Specimens from the pancreas were processed for light, electron microscopy and pancreatic insulin mRNA expression. Blood samples were assayed for the levels of glucose, insulin, and total peroxides. In the atRA-treated group, the number of the islets and the islet area significantly increased. Strong insulin-immunoreactive endocrine-like cells were observed nearby the pancreatic acini and the interlobular ducts. Interestingly, insulin-positive cells seemed to arise from pancreatic acinar and ductal epithelium. Ultrastructurally, beta-cells, acinar, and ductal cells restored their normal appearance. Pancreatic insulin mRNA and blood indices were almost normalized. AtRA improved the histological changes of the pancreas and the blood indices in diabetic rats. (C) 2015 Wiley Periodicals, Inc.