Is it absent or is it present? Detection of a non-native fish to inform management decisions using a new highly-sensitive eDNA protocol
Is it absent or is it present? Detection of a non-native fish to inform management decisions using a new highly-sensitive eDNA protocol
复制标题
它是不存在还是存在？
作者：
P. I. Davison;Mathilde Falcou;G. Copp;G. Davies;L. Vilizzi;V. Créach
环境管理人员需要一种敏感和可靠的手段，以尽可能高的置信度证明哪里有非本地鱼种，哪里没有。因此，巢式PCR（nPCR）协议的开发，以检测环境DNA（eDNA）的案例研究物种，麦穗鱼麦穗鱼，这是最近的主题，在英国的国家根除运动。在英格兰南部的一个商业垂钓场所，在实验室和现场进行了一系列协调调查（eDNA和常规陷阱采样），对nPCR方案进行了测试，其中基于常规PCR（cPCR）的初始eDNA调查发现，P. parva存在于七个池塘之一。在实验室中，nPCR方案的灵敏度平均比cPCR高100倍，在DNA浓度为3 × 10−8 ng/µL（每µL 8个DNA拷贝）时，检测率为100%。在现场，nPCR和常规诱捕都只在七个垂钓池塘中的一个中检测到P. parva，与先前基于cPCR的研究中相同的受感染池塘。在对受侵扰的池塘进行根除工作后，使用nPCR在先前受侵扰的池塘或邻近的池塘中均未检测到P. parva的eDNA，该池塘已用于检疫大型商业价值鱼类。在管理应用中，阴性结果的准确性可能与阳性检测的确认同等重要，nPCR协议为现有的分析工具包提供了有用的补充，可为负责非本地物种管理的决策者提供信息。
Environmental managers require a sensitive and reliable means to prove, with the highest level of confidence possible, where non-native fish species exist and where they do not. Therefore, a nested PCR (nPCR) protocol was developed to detect the environmental DNA (eDNA) of a case-study species, topmouth gudgeon Pseudorasbora parva, which was recently the subject of a national eradication campaign in the UK. The nPCR protocol was tested in the laboratory and in the field in a series of coordinated surveys (eDNA and conventional sampling with traps) at a commercial angling venue in southern England where an initial eDNA survey, based on conventional PCR (cPCR), found P. parva to be present in one of the seven ponds. In the laboratory, the nPCR protocol was on average 100× more sensitive than cPCR, providing a 100% detection rate at DNA concentrations of 3 × 10−8 ng/µL (8 DNA copies per µL). In the field, nPCR and conventional trapping both detected P. parva in only one of the seven angling ponds, the same infested pond as in the previous cPCR-based study. Following eradication work on the infested pond, no eDNA of P. parva was detected using nPCR in either the formerly-infested pond or the adjacent pond, which had been used to quarantine large commercially-valuable fishes. In management applications where the veracity of negative results may be of equal importance as confirmation of positive detections, nPCR protocols provide a useful addition to the analytical toolkit available to inform decision makers responsible for non-native species management.
影响因子：
3.8
作者：
Godornes, Charmie;Leader, Brandon Troy;Lukehart, Sheila A.
通讯作者：
Lukehart, Sheila A.