In Vitro Diagnosis of Malignant Hyperthermia Susceptibility with Ryanodine-Induced Contractures in Human Skeletal Muscles
In Vitro Diagnosis of Malignant Hyperthermia Susceptibility with Ryanodine-Induced Contractures in Human Skeletal Muscles
复制标题
瑞尼定引起的人体骨骼肌挛缩对恶性高热敏感性的体外诊断
DOI：
--
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发表时间：
1996
影响因子：
5.7
通讯作者：
J. S. am Esch
中科院分区：
文献类型：
--
作者：
F. Wappler;N. Roewer;A. Kochling;J. Scholz;M. Steinfath;J. S. am Esch
The in vitro contracture test with ryanodine is a new method to distinguish malignant hyperthermia (MH) susceptible (MHS) from normal (MHN) patients.The purpose of our investigation was to determine whether smaller concentrations of ryanodine than those used previously may result in a better differentiation. We performed a ryanodine contracture test (RCT) using concentrations of 1 and 2 micro Meter in muscle specimens of 41 MHS, 58 MHN, and 19 MH-equivocal (MHE) patients. Nine patients were excluded from the study due to neuromuscular diseases. All contracture levels (i.e., start of contractures, contractures of 0.2 g and 1.0 g) were attained significantly earlier in MHS than in MHN muscles at both concentrations of ryanodine. Using a ryanodine concentration of 2 micro Meter, all contracture levels were reached significantly faster than with 1 micro Meter. There was no overlap in the range of times between groups at all contracture levels with ryanodine 1 and 2 micro Meter. The median threshold times for all MHE patients were always between those of MHS and MHN. Defining arbitrarily threshold times for MHS and MHN, an assignment of MHE patients to either MHS or MHN using 1 or 2 micro Meter ryanodine was possible in most cases. Ryanodine administration at a concentration of 1 micro Meter led to a better distinction of MHS from MHN patients than 2 micro Meter. The RCT with ryanodine 1 micro Meter should therefore be added to the current diagnostic methods. (Anesth Analg 1996;82:1230-6)
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影响因子：
3.5
作者：
Iles,DE;Lehmann-Horn,F;Scherer,SW;Tsui,LC;OldeWeghuis,D;Suijkerbuijk,RF;Heytens,L;Mikala,G;Schwartz,A;Ellis,FR
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影响因子：
5.7
作者：
Wedel,DJ;Nelson,TE
通讯作者：
Nelson,TE
恶性高热易感位点 (MHS2) 定位于人类染色体 17q 的证据。
影响因子：
4.4
作者：
Levitt,RC;Olckers,A;Meyers,S;Fletcher,JE;Rosenberg,H;Isaacs,H;Meyers,DA
通讯作者：
Meyers,DA
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影响因子：
9.8
作者：
Fletcher,JE;Tripolitis,L;Hubert,M;Vita,GM;Levitt,RC;Rosenberg,H
通讯作者：
Rosenberg,H
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mickelson,JR;Gallant,EM;Litterer,LA;Johnson,KM;Rempel,WE;Louis,CF
通讯作者：
Louis,CF