QUANTITATIVE-ANALYSIS OF MOLECULES WHICH DISTINGUISH FUNCTIONAL COMPARTMENTS WITHIN GERMINAL-CENTERS
QUANTITATIVE-ANALYSIS OF MOLECULES WHICH DISTINGUISH FUNCTIONAL COMPARTMENTS WITHIN GERMINAL-CENTERS
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DOI：
10.1002/eji.1830230502
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发表时间：
1993-05-01
影响因子：
5.4
通讯作者：
MACLENNAN, ICM
中科院分区：
文献类型：
--
作者：
HARDIE, DL;JOHNSON, GD;MACLENNAN, ICM
本文描述了人扁桃体次级滤泡的五个区带，并根据其组成细胞的表型加以区分。从卵泡顶端到卵泡底部，这些区域被称为：卵泡膜、外区、顶端亮区、基底区和暗区。暗区包括增殖、CD77高表达、中心母细胞和稀疏分布的滤泡树突状细胞(FDC)。另一方面，顶端和基底区有密集的FDC网络，表达CD21和CD54。根尖光区的FDC与基底区的FDC不同，主要是CD23的高表达。暗区的中心母细胞产生非增殖性的中心细胞，这些中心细胞在光区顶部移动(EUR。J.免疫。1991年。21：2951)。基底光区的中心细胞比根尖光区的中心细胞更嗜派洛宁，排列更紧密，体积更大。因此，根据传统的组织学，基底区似乎是暗区的一部分。然而，采用的命名法遵循了暗区充满增殖的中心母细胞的惯例。在暗区和亮区均可观察到细胞的凋亡，但其中一半以上的细胞位于基底区。这与在暗区经历免疫球蛋白可变区基因体细胞突变的细胞的后代迁移到亮区的概念是一致的，在光明区，根据它们与FDC上的抗原结合的能力而被选择。接受抗原依赖信号的细胞存活，而那些不会通过凋亡自杀的细胞。外区不含CD23高表达细胞，与邻近的卵泡套膜和根尖光区不同。它含有小淋巴样细胞、母细胞和浆细胞样细胞。外区许多细胞强烈表达CDw75。外层区域也以窄带的形式围绕着暗区延伸。光区和外区FDC和T细胞在诱导中心细胞中的可能作用对分化为记忆细胞或成浆细胞进行了讨论。
Five zones in the secondary follicles of human tonsils are described, which are distinguished by the phenotype of their constituent cells. Moving from the apex to the base of the follicle the zones are termed: follicular mantle, outer zone, apical light zone, basal light zone and dark zone. The dark zone contains proliferating, CD77high, centroblasts and thin, widely spaced processes of follicular dendritic cells (FDC). The apical and basal light zones on the other hand contain a dense network of FDC which express CD21 and CD54 strongly. The FDC of the apical light zone differ from those of the basal light zone by their high expression of CD23. Centroblasts of the dark zone give rise to non-proliferating centrocytes which move apically through the light zone (Eur. J. Immunol. 1991. 21: 2951). The centrocytes of the basal light zone are more pyroninophilic, more closely-packed and larger than those in the apical light zone. Consequently, by conventional histology the basal light zone appears to be part of the dark zone. The nomenclature adopted, however, adheres to the convention that the dark zone is filled with proliferating centroblasts. Cells undergoing apopotosis were identified both in the dark and light zones, but more than half of these cells were located in the basal light zone. This is consistent with the concept that the progeny of cells which undergo somatic mutation in their immunoglobulin variable region genes in the dark zone migrate to the light zone where they are selected on the basis of their capacity to bind to antigen held on FDC. Cells receiving an antigen-dependent signal survive while those that do not kill themselves by apoptosis. The outer zone does not contain CD23high cells and in this way is distinguished from the adjacent follicular mantle and apical light zone. It contains small lymphoid cells, blasts and plasmacytoid cells. Many cells of the outer zone express CDw75 strongly. The outer zone also extends as a narrow band around the dark zone. Possible roles of FDC and T cells of the light zone and outer zone in inducing centrocytes. to differentiate to memory cells or plasmablasts are discussed.