Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.
Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.
复制标题
使用出芽酵母中的条件表达分析减数分裂染色体相关蛋白动力学。
DOI：
10.1007/978-1-4939-6340-9_8
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Rockmill,Beth
中科院分区：
文献类型：
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作者：
MacQueen,AmyJ;Rockmill,Beth
在野生型和突变细胞中减数分裂染色体及其相关蛋白质结构的可视化为生殖细胞形成的分子途径提供了有价值的见解。在这里，我们描述的基本方法，可视化减数分裂染色体在一个长期的模式生物，研究分子和细胞生物学的减数分裂，芽殖酵母，S。啤酒。本章还强调了各种条件表达制度，可用于了解动态和/或染色体蛋白质结构的发育限制，这种动态方面的大分子结构，介导减数分裂染色体生物学通常是不明显的标准蛋白质可视化实验。
The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast,S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.