Association patterns of Pseudomonas aeruginosa clinical isolates as revealed by virulence traits, antibiotic resistance, serotype and genotype
Association patterns of Pseudomonas aeruginosa clinical isolates as revealed by virulence traits, antibiotic resistance, serotype and genotype
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DOI：
10.1111/j.1574-695x.2007.00328.x
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Tenreiro, Rogerio
中科院分区：
文献类型：
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作者：
Fonseca, Antonio Pedro;Correia, Petra;Tenreiro, Rogerio
本研究的目的是使用分层聚类分析评估96株铜绿假单胞菌临床分离株的关联模式，这些数据来自测量理化细胞表面特性、粘附和初始生物膜形成能力获得的数据，以研究与来源、血清型、β-内酰胺模式、运动性和M13-PCR基因组或克隆谱系的任何对应关系，以及选择可以作为来自葡萄牙中心医院的该铜绿假单胞菌群体的基因型和表型多样性的代表的临床分离株。分离株的表型特征在于其粘附和形成聚苯乙烯表面上的生物膜的能力，其对十六烷和硅酮的亲和力，其游泳和抽搐能力，其抗生素敏感性模式和其血清型。未发现与同一来源、血清型、β-内酰胺酶谱、运动性、M13-PCR基因组和克隆谱系相关的特定表型簇，但在低遗传相似性基础上筛选出的5株代表性铜绿假单胞菌也分散在表型簇中。
The aims of this study were to assess the association patterns of 96 clinical isolates of Pseudomonas aeruginosa using hierarchical cluster analysis from data obtained from the measurement of the physicochemical cell surface properties, adhesion and initial biofilm formation abilities, to investigate any correspondence with source, serotype, beta-lactam pattern, motility and M13-PCR genogroup or clonal lineage, as well as to select clinical isolates that could act as representatives of the genotypic and phenotypic diversity of this P. aeruginosa population from a Portuguese Central Hospital. The isolates were phenotypically characterized by their ability to adhere and form biofilms on polystyrene surfaces, their affinity to hexadecane and silicone, their swimming and twitching abilities, their antibiotic susceptibility patterns and their serotypes. No particular phenotypic cluster associated with the same source, serotype, beta-lactam pattern, motility and M13-PCR genogroup and clonal lineage was found. Nevertheless, five representative strains of the P. aeruginosa population from this Hospital, selected on the basis of low genetic similarity, were also found to be dispersed among the phenotypic clusters.