Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS
Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS
复制标题
使用 UHPLC-ESI-MS/MS 定量分析冷应激诱导腐败希瓦氏菌脂质组学变化
DOI：
10.3390/molecules24244609
复制
发表时间：
2019-12-01
期刊：
影响因子：
4.6
通讯作者：
Xie, Jing
中科院分区：
文献类型：
--
作者：
Gao, Xin;Liu, Wenru;Xie, Jing
腐败希瓦氏菌（Shewanellaputrefaciens）是一种常见的冷藏鲜鱼腐败菌和耐寒微生物。冷适应机制包括通过精细调节脂质组成的能力增加脂质膜的流动性。在本研究中，S。采用超高压液相色谱/电喷雾串联质谱（UHPLC-ESI-MS/MS）技术，对30、20、10、4、0 ℃培养的腐败菌进行了研究，探讨了脂质组成对低温耐受性的影响。脂质组学分析共检测到27种脂质，606种脂质分子。在30、20、10、4和0 ℃下培养的腐败菌。S. 30 ℃培养的腐殖菌（SP-30）中的甘油脂、鞘脂、糖脂和脂肪酸的含量显著高于0 ℃培养的腐殖菌（SP-0），但磷脂的含量也较低（13.97%）。PE（30：0）、PE（15：0/15：0）、PE（31：0）、PA（33：1）、PE（32：1）、PE（33：1）、PE（25：0）、PC（22：0）、PE（29：0）、PE（34：1）、dMePE（15：0/16：1）、PE（31：1）、dMePE（15：1/15：0）、PG（34：2）和PC（11：0/11：0）被鉴定为S中最丰富的脂质分子种类。在30、20、10、4和0 ℃下培养的腐败菌。PG含量的增加有助于膜脂双分子层的构建，成功地维持了低温胁迫下膜的完整性。S.低温培养的腐败菌显著增加了总不饱和液体含量，但降低了饱和液体含量。
Shewanella putrefaciens is a well-known specific spoilage organism (SSO) and cold-tolerant microorganism in refrigerated fresh marine fish. Cold-adapted mechanism includes increased fluidity of lipid membranes by the ability to finely adjust lipids composition. In the present study, the lipid profile of S. putrefaciens cultivated at 30, 20, 10, 4, and 0 degrees C was explored using ultra-high-pressure liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) to discuss the effect of lipid composition on cold-adapted tolerance. Lipidomic analysis detected a total of 27 lipid classes and 606 lipid molecular species in S. putrefaciens cultivated at 30, 20, 10, 4, and 0 degrees C. S. putrefaciens cultivated at 30 degrees C (SP-30) had significantly higher content of glycerolipids, sphingolipids, saccharolipids, and fatty acids compared with that at 0 degrees C (SP-0); however, the lower content of phospholipids (13.97%) was also found in SP-30. PE (30:0), PE (15:0/15:0), PE (31:0), PA (33:1), PE (32:1), PE (33:1), PE (25:0), PC (22:0), PE (29:0), PE (34:1), dMePE (15:0/16:1), PE (31:1), dMePE (15:1/15:0), PG (34:2), and PC (11:0/11:0) were identified as the most abundant lipid molecular species in S. putrefaciens cultivated at 30, 20, 10, 4, and 0 degrees C. The increase of PG content contributes to the construction of membrane lipid bilayer and successfully maintains membrane integrity under cold stress. S. putrefaciens cultivated at low temperature significantly increased the total unsaturated liquid contents but decreased the content of saturated liquid contents.