Transplantation of retroviral producer cells for in vivo gene transfer into mouse skeletal muscle.
Transplantation of retroviral producer cells for in vivo gene transfer into mouse skeletal muscle.
复制标题
移植逆转录病毒生产细胞，将体内基因转移到小鼠骨骼肌中。
作者：
A. Fassati;D. Wells;F. Walsh;G. Dickson
我们描述了一种新的策略，有效地在体内基因转移到骨骼肌逆转录病毒载体。将先前用细胞抑制药物丝裂霉素C处理的重组逆转录病毒生产细胞注射到成年裸小鼠、裸/mdx小鼠和C57 BL/10小鼠的再生肌肉中。使用LacZ报告基因活性，我们在所有小鼠品系中检测到有效的转导（裸，平均11%，范围4.2-21%; C57 BL/10，平均12%，范围3.4-20%;裸mdx，注射后4周时平均4.3%，范围2.1-7%，注射后12周时6.6%，范围1.3-12%）。外源基因表达维持在高水平至少3个月。这种策略允许肌肉卫星细胞在体内转染，形成转基因的储存库，用于在随后的变性和再生中掺入新的肌纤维中。由于其效率和潜在的广泛应用，该程序代表了骨骼肌和其他组织中体内遗传转移的新策略。
We describe a new strategy for efficient in vivo gene transfer into skeletal muscle using retroviral vectors. Recombinant retroviral producer cells, previously treated with the cytostatic drug mitomycin C, were injected into regenerating muscle of adult nude, nude/mdx, and C57BL/10 mice. Using LacZ reporter gene activity, we detected efficient transduction in all mouse strains (Nude, mean 11%, range 4.2-21%; C57BL/10, mean 12%, range 3.4-20%; Nude mdx, mean 4.3%, range 2.1-7% at 4 weeks post-injection and 6.6%, range 1.3-12% at 12 weeks post-injection). Foreign gene expression was sustained at high levels for at least 3 months. This strategy allows muscle satellite cells to be transfected in vivo, forming a reservoir of the transgene for incorporation into new myofibers in subsequent rounds of degeneration and regeneration. Because of its efficiency and potentially broad application, this procedure represents a new strategy for in vivo genetic transfer in skeletal muscle and potentially in other tissues.
通过在培养皿中与 β-半乳糖苷酶底物一起孵育细胞来进行 DNA 转染的简单测定。
影响因子：
2.7
作者：
Lim,K;Chae,CB
通讯作者：
Chae,CB
循环排列转移 RNA 的折叠。
DOI：
10.1126/science.1720569
发表时间：
1991
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pan,T;Gutell,RR;Uhlenbeck,OC
通讯作者：
Uhlenbeck,OC
DOI：
10.1073/pnas.88.4.1330
发表时间：
1991-02-01
影响因子：
11.1
作者：
PALMER, TD;ROSMAN, GJ;MILLER, AD
通讯作者：
MILLER, AD