An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-D-glucose and 3-O-β-gentiobiosyl-D-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively
An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-D-glucose and 3-O-β-gentiobiosyl-D-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively
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构巢曲霉内-β-1,3-葡聚糖酶表现出特定的催化特性，用于从大麦中制备具有高抗氧化活性的 3-O-β-纤维二糖基-d-葡萄糖和 3-O-β-龙胆二糖基-d-葡萄糖
DOI：
10.1016/j.ijbiomac.2021.07.053
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发表时间：
2021-07-13
影响因子：
8.2
通讯作者：
Yuan, Sheng
中科院分区：
文献类型：
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作者：
Liu, Cuicui;Yan, Songling;Yuan, Sheng
来自构巢曲霉（Aspergillus nidulans）的内切β-1，3（4）-葡聚糖酶AnENG 16 A显示出独特的β-葡聚糖水解催化特征。AnENG 16 A水解双环昆布多糖主要产生3-O-β-龙胆二糖基-D-葡萄糖，水解大麦β-葡聚糖主要产生3-O-β-纤维二糖基-D-葡萄糖。采用分子排阻色谱法从AnENG 16 A大麦β-葡聚糖水解液和E.双环海带多糖进一步研究表明，3-O-β-纤维二糖基-D-葡萄糖的抗氧化活性比大麦β-葡聚糖高8.99倍，3-O-β-龙胆二糖基-D-葡萄糖的抗氧化活性比E.双环海带多糖值得注意的是，3-O-β-纤维二糖基-D-葡萄糖和3-O-β-龙胆二糖基-D-葡萄糖表现出的抗氧化活性分别比昆布三糖高148.9%和116.0%，表明β-葡萄糖三糖的非还原端的β-1，4-键或β-1，6-键比β-1，3-键具有增强抗氧化活性的作用。此外，3-O-β-纤维二糖基-D-葡萄糖的抗氧化活性比纤维三糖高237.9%，海带多糖的抗氧化活性比大麦β-葡聚糖高5.06倍，表明β-葡聚糖或寡糖还原端的β-1，4-键导致抗氧化活性比β-1，3-键降低。
An endo-beta-1,3(4)-glucanase AnENG16A from Aspergillus nidulans shows distinctive catalytic features for hydrolysis of beta-glucans. AnENG16A hydrolyzed Eisenia bicyclis laminarin to mainly generate 3-O-beta gentiobiosyl-D-glucose and hydrolyzed barley beta-glucan to mainly produce 3-O-beta cellobiosyl-D-glucose. Using molecular exclusion chromatography, we isolated and purified 3-O-beta cellobiosyl-D-glucose and 3-O-beta-gentiobiosyl-D-glucose, respectively, from AnENG16A-hydrolysate of barley beta-glucan and E. bicyclis laminarin. Further study reveals that 3-O-beta-cellobiosyl-D-glucose had 8.99 fold higher antioxidant activity than barley p glucan and 3-O-beta-gentiobiosyl-D-glucose exhibited 43.0% higher antioxidant activity than E. bicyclis laminarin. Notably, 3-O-beta-cellobiosyl-D-glucose and 3-O-beta-gentiobiosyl-D-glucose exhibited 148.9% and 116.0% higher antioxidant activity than laminaritriose, respectively, indicating that beta-1,4-linkage or -1,6-linkage at non-reducing end of beta-glucotrioses had enhancing effect on antioxidant activity compared to beta-1,3-linkage. Furthermore, 3-O-beta-cellobiosyl-D-glucose showed 237.9% higher antioxidant activity than cellotriose, and laminarin showed 5.06-fold higher antioxidant activity than barley beta-glucan, indicating that beta-1,4-linkage at reducing end of beta-glucans or oligosaccharides resulted in decrease of antioxidant activity compared to beta-1,3-linkage.