A study of communication specificity between cells in culture
A study of communication specificity between cells in culture
复制标题
培养细胞间通讯特异性的研究
作者：
M. Epstein;N. Gilula
我们已经研究了在文化中的细胞之间的通信的特异性，通过共培养细胞来自哺乳动物，鸟类和节肢动物的有机体。哺乳动物和禽类培养细胞具有相似的缝隙连接表型，而昆虫（节肢动物）细胞系具有显著不同的缝隙连接结构。电生理和超微结构的方法被用来检查离子耦合和连接之间的相互作用同源和异源细胞类型。在同源细胞系统中，间隙连接和离子偶联以高发生率存在。此外，共培养的异源脊椎动物细胞可以容易地进行交流。相比之下，在异源昆虫细胞系（同翅目或鳞翅目）和脊椎动物细胞（哺乳动物心肌细胞或3 T3细胞）之间几乎检测不到偶联（0-8%）。在节肢动物和脊椎动物细胞类型之间没有观察到间隙连接，即使异源细胞可以分离小于10 nm。在另外的研究中，发现来自不同节肢动物目的异源昆虫细胞系之间偶联的发生率较低。然而，广泛的耦合之间检测到的昆虫细胞系，来自同一目的（同翅目）。这些观察结果表明，在表达相同间隙连接表型的培养物中脊椎动物细胞之间的通信几乎没有或没有明显的特异性，而在培养物中节肢动物细胞之间存在明确的通信特异性。
We have examined the specificity of communication between cells in culture by co-culturing cells derived from mammalian, avian, and arthropod organisms. Both mammalian and avian culture cells have similar gap junctional phenotypes, while the insect (arthropod) cell lines have a significantly different gap junctional structure. Electrophysiological and ultrastructural methods were used to examine ionic coupling and junctional interactions between homologous and heterologous cell types. In homologous cell systems, gap junctions and ionic coupling are present at a high incidence. Also, heterologous vertebrate cells in co-culture can communicate readily. By contrast, practically no coupling (0-8%) is detectable between heterologous insect cell lines (Homopteran or Lepidopteran) and vertebrate cells (mammalian myocardial or 3T3 cells). No gap junctions have been observed between arthropod and vertebrate cell types, even though the heterologous cells may be separated by less than 10 nm. In additional studies, a low incidence of coupling was found between heterologous insect cell lines derived from different arthropod orders. However, extensive coupling was detected between insect cell lines that are derived from the same order (Homoptera). These observations suggest that there is little or no apparent specificity for communication between vertebrate cells in culture that express the same gap junctional phenotype, while there is a definite communication specificity that exists between arthropod cells in culture.