DEVELOPMENT OF METATARSOPHALANGEAL JOINT OF CHICK-EMBRYO - MORPHOLOGICAL, ULTRASTRUCTURAL AND HISTOCHEMICAL STUDIES
DEVELOPMENT OF METATARSOPHALANGEAL JOINT OF CHICK-EMBRYO - MORPHOLOGICAL, ULTRASTRUCTURAL AND HISTOCHEMICAL STUDIES
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DOI：
10.1002/aja.1001500207
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
MITROVIC, DR
中科院分区：
文献类型：
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作者：
MITROVIC, DR
应用组织化学方法，对孵化至第15天的鸡胚胎发育过程中的组织发生和关节裂解机制进行了研究。关节组织分化后24小时仍可见细胞变性，推测这种早期细胞坏死可能是关节间充质内侧部分松动导致3层胚胎关节分化的原因。关节裂解时，在发育中的关节腔周围也可见退变细胞。在某些情况下，在分裂之前和/或伴随着沿着组织分裂区紧密排列的细长的、嗜碱性的和不透明电子的细胞的出现。这些细胞可能以某种方式牵连在分裂过程中，后来构成关节软骨的表层细胞层。在原始滑液中发现了明确的形态和组织放射自显影证据，证明存在有机成分，可能是粘多糖。体液分泌也可能是积液部位组织分裂的原因。关节腔的形成是内在的、遗传的和外在的力学因素共同作用的结果。
The histogenesis and mechanism of joint clefting of the developing chick embryo up to the 15th day of incubation were studied morphologically, ultrastructurally and by histochemical methods. Cell degeneration was consistently noted 24 h after differentiation of the joint tissue, and it is postulated that this early cell necrosis may account for the loosening of the medial part of articular mesenchyme (interzone) leading to differentiation of a 3-layered embryonic joint. At the time of joint clefting degenerative cells were also seen in the peripheral parts of the developing articular cavity. In some cases clefting was immediately preceded and/or accompanied by the appearance of elongated, basophilic and electron-opaque cells closely arranged along the zone of tissue cleavage. These cells may be implicated in some way in the clefting process and later to constitute a surface cell layer of articular cartilage. Clear morphological and histoautoradiographic evidence was found for the presence of an organic component, presumably mucopolysaccharide, in the primitive synovial fluid. Fluid secretion may also account for tissue cleavage at the sites of its accumulation. Joint cavity formation resulted from a combination of both intrinsic, genetically expressed and extrinsic mechanical factors acting synchronously.