IMPROVED IDENTIFICATION OF METHANOGENIC BACTERIA BY FLUORESCENCE MICROSCOPY

IMPROVED IDENTIFICATION OF METHANOGENIC BACTERIA BY FLUORESCENCE MICROSCOPY
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DOI:
10.1128/aem.36.5.752-754.1978
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发表时间:
1978-01-01
影响因子:
4.4
通讯作者:
VOGELS, GD
VOGELS, GD
中科院分区:
生物学2区
文献类型:
--
作者:
DODDEMA, HJ;VOGELS, GD

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产甲烷菌可以通过荧光显微镜初步鉴定。通过仔细选择与产甲烷细菌中一些独特的辅酶F420和F350的激发和发射光谱相匹配的一系列激发和屏障过滤器,改进了该技术。[巴氏甲烷八叠球菌,甲酸甲烷杆菌,甲烷杆菌菌株M.o.H.,使用亨氏甲烷杆菌、甲烷杆菌菌株TH、嗜热甲烷杆菌和反刍甲烷杆菌。
Methanogenic bacteria can be tentatively identified by fluorescence microscopy. This technique was improved by carefully selecting a series of excitation and barrier filters that matched the excitation and emission spectra of some unique coenzymes, F420 and F350, in methanogenic bacteria. [Methanosarcina barkeri, Methanobacterium formicicum, Methanobacterium strain M.o.H., Methanospirillum hungatii, Methanobacterium strain TH, Methanobacterium thermoautrophicum and Methanobacterium ruminantium were used.].