Staphylococcus aureus peptidoglycan is a Toll-like receptor 2 activator:: a reevaluation
Staphylococcus aureus peptidoglycan is a Toll-like receptor 2 activator:: a reevaluation
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DOI：
10.1128/iai.73.8.5212-5216.2005
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发表时间：
2005-08-01
影响因子：
3.1
通讯作者：
Gupta, D
中科院分区：
文献类型：
--
作者：
Dziarski, R;Gupta, D
由于肽聚糖（PGN）激活Toll样受体2（TLR2）的能力最近受到质疑，我们重新评估PGN激活TLR2。聚合物可溶性或不溶性金黄色葡萄球菌PGN，再纯化十二烷基硫酸钠或苯酚提取，激活TLR2在0.1至1或10 μ g/ml，分别诱导肿瘤坏死因子α的生产。溶菌酶消化可消除PGN对TLR2的激活作用，但脂磷壁酸对TLR2的激活作用无影响。我们得出结论，聚合S。aureus PGN是TLR 2激活剂。
Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 mu g/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.