Extragonadal oocytes residing in the mouse ovarian hilum contribute to fertility.
Extragonadal oocytes residing in the mouse ovarian hilum contribute to fertility.
复制标题
存在于小鼠卵巢门中的性腺外卵母细胞有助于生育。
DOI：
10.1095/biolreprod.116.145631
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发表时间：
2017
影响因子：
3.6
通讯作者：
DeFalco,Tony
中科院分区：
文献类型：
--
作者：
Sun,Xiaofei;Ito,Junya;Potter,SarahJ;Dey,SudhansuK;DeFalco,Tony
对受体和供体小鼠卵巢切除后卵巢移植所生幼仔的观察提出了卵母细胞卵巢外来源的有趣可能性。然而，哺乳动物成熟卵母细胞是否存在于性腺外组织中仍然是一个谜。使用转基因荧光报告小鼠和移植手术，我们证明了存在的捐助者和捐赠者衍生的黄体和恢复的捐助者和捐赠者衍生的后代从卵巢切除小鼠移植后的捐助者卵巢。卵巢外卵母细胞的一个潜在区域是卵门，它是卵巢和生殖道之间的韧带样结构。小鼠卵巢和生殖道的免疫荧光共聚焦显微镜显示，原始卵泡的人口居住在卵巢外的门内。仅卵巢切除的对照组证实，手术后卵母细胞仍留在受体门中。这些结果提供了证据表明，门是卵泡的储备来源，这些卵泡可能返回卵巢成熟和排卵。通过确定一个新的卵泡储库，我们的研究解决了生殖生物学中一个长期存在的问题，并有助于对卵巢功能和生育能力的新概念知识。
The observation of pups born from recipient and donor mice after ovariectomy followed by ovarian transplant poses the interesting possibility of an extraovarian source of oocytes. However, whether mammalian adult oocytes reside in extragonadal tissues remains elusive. Using transgenic fluorescent reporter mice and transplantation surgeries, we demonstrate the presence of both donor- and recipient-derived corpora lutea and recovery of both donor- and recipient-derived offspring from ovariectomized mice after transplantation of donor ovaries. A potential region for extraovarian oocytes is the hilum, a ligament-like structure between the ovary and the reproductive tract. Immunofluorescent confocal microscopy of mouse ovaries and reproductive tracts revealed that a population of primordial follicles resides outside the ovary within the hilum. Ovariectomy-only controls confirmed that oocytes remain in the recipient hilum after surgery. These results provide evidence that the hilum is a reserve source of follicles, which likely return to the ovary for maturation and ovulation. By identifying a new follicle reservoir, our study addresses a long-standing question in reproductive biology and contributes to new conceptual knowledge about ovarian function and fertility.