Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
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DOI：
10.1016/s0887-2333(01)00108-4
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发表时间：
2002-04-01
影响因子：
3.2
通讯作者：
Zierold, K
中科院分区：
文献类型：
--
作者：
Bolkent, S;Zierold, K
用电子探针X射线显微分析法研究了离子载体缬氨霉素、离子霉素和短杆菌肽A对培养大鼠肝细胞的元素区室化作用。这些离子载体的最显著的作用是细胞内钾/钠比从对照条件下的约10的值降低到应用离子载体后的低于1的值。钠、钾、氯在细胞质和细胞核中的变化相似。然而，相对于周围的细胞质，线粒体中的元素变化被延迟或阻碍。细胞质和线粒体的含水量略有增加。除此之外，每种离子载体对细胞内离子分布都有特定的影响。与短杆菌肽A和离子霉素相比，缬氨霉素不改变细胞内氯含量。离子霉素诱导线粒体中的钙积累。研究的离子载体对细胞内元素分布的细胞毒性效应比从它们在膜中的离子选择性所假设的更复杂。(C)2002爱思唯尔科技有限公司版权所有。
The element compartmentation in cultured rat hepatocytes was studied by electron probe X-ray microanalysis of freeze-dried cryosections after exposure of the cells to the ionophores valinomycin, ionomycin or gramicidin A. The most striking effect of these ionophores is the decrease of the intracellular potassium/sodium ratio from values of approximately 10 under control conditions to values below I after application of the ionophores. Changes of sodium, potassium and chloride are similar in cytoplasm and nucleus. However, elemental changes are delayed or impeded in mitochondria with respect to the surrounding cytoplasm. The water portion of cytoplasm and mitochondria slightly increases. Besides that, each ionophore has specific effects on the intracellular ion distribution. As compared to gramicidin A and ionomycin, valinomycin does not change the intracellular chloride content. lonomycin induces calcium accumulation in mitochondria. The cytotoxic effects of the studied ionophores on the intracellular element distribution are more complex than supposed from their ion selective properties in membranes. (C) 2002 Elsevier Science Ltd. All rights reserved.