The distribution and time-dependent expression of MAGL during skeletal muscle wound healing in rats.
The distribution and time-dependent expression of MAGL during skeletal muscle wound healing in rats.
复制标题
大鼠骨骼肌伤口愈合过程中 MAGL 的分布和时间依赖性表达。
DOI：
10.14670/hh-11-625
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发表时间：
2015-04
影响因子：
2
通讯作者：
Guan, Da-Wei
中科院分区：
文献类型：
--
作者：
Li, Shan-Shan;Liu, Min;Wang, Meng;Guan, Da-Wei
单酰基甘油脂肪酶（MAGL）广泛分布于哺乳动物体内，主要负责2-花生四烯醇甘油（2-AG）的代谢。其在创伤后骨骼肌中的表达尚不清楚。采用免疫组化染色、Western blotting和定量实时荧光定量PCR （qPCR）方法对大鼠骨骼肌创面愈合过程中MAGL的表达和分布进行了时间依赖性研究。采用Sprague-Dawley雄性大鼠40只，建立骨骼肌挫伤动物模型。分别于伤后1、3、5、7、9、13、17、21天取标本，每间隔5只。取5只大鼠作为对照。在对照大鼠肌纤维肌浆中观察到MAGL的弱免疫反应性。MAGL在损伤组织的多形核细胞（PMNs）、圆形单核细胞（MNCs）、纺锤形成纤维细胞（FBCs）和再生的多核肌管中观察到强烈的免疫反应。随后，中性粒细胞、巨噬细胞和肌成纤维细胞被双重免疫荧光鉴定为magl阳性细胞。经qPCR和Western blot分析，损伤后MAGL的表达明显上调。结果表明，创伤后骨骼肌中MAGL的表达具有一定的细胞类型和时间依赖性，提示骨骼肌创伤愈合过程中MAGL可能参与了炎症反应、纤维生成和肌肉再生。
Monoacylglycerol lipase (MAGL) is widely distributed in mammals and largely responsible for metabolizing 2-arachidonoylglycerol (2-AG). Little is known about its expression in skeletal muscles after trauma. A preliminary study on time-dependent expression and distribution of MAGL was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR (qPCR) during skeletal muscle wound healing in rats. An animal model of skeletal muscle contusion was established in 40 Sprague-Dawley male rats. Samples were taken at 1, 3, 5, 7, 9, 13, 17 and 21 days after contusion, respectively (5 rats in each posttraumatic interval). 5 rats were employed as control. Weak immunoreactivity of MAGL was observed in the sarcoplasm of myofibers in control rats. Intensive immunoreactivities of MAGL were observed in polymorphonuclear cells (PMNs), round-shaped mononuclear cells (MNCs), spindle-shaped fibroblastic cells (FBCs) and regenerated multinucleated myotubes in the injured tissue. Subsequently, neutrophils, macrophages and myofibroblasts were identified as MAGL-positive cells by double immunofluorescent procedure. MAGL expression was remarkably up-regulated after contusion by qPCR and Western blot analysis. The results demonstrate that the expression of MAGL is distributed in certain cell types and time-dependently expressed in skeletal muscles after trauma, suggesting that MAGL may be involved in inflammatory response, fibrogenesis and muscle regeneration during skeletal muscle wound healing.