Elevated gamma-aminobutyric acid, glutamate, and vascular endothelial growth factor levels in the vitreous of patients with proliferative diabetic retinopathy
Elevated gamma-aminobutyric acid, glutamate, and vascular endothelial growth factor levels in the vitreous of patients with proliferative diabetic retinopathy
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DOI：
10.1001/archopht.1997.01100160331011
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发表时间：
1997-09-01
影响因子：
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通讯作者：
Ambati, BK
中科院分区：
文献类型：
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作者：
Ambati, J;Chalam, KV;Ambati, BK
目的：检测非糖尿病和糖尿病患者玻璃体中γ-氨基丁酸（GABA）、谷氨酸和血管内皮生长因子（VEGF）的相对水平。方法：从22例增殖性糖尿病视网膜病变（PDR）患者和28例接受平坦部玻璃体切除术的非糖尿病患者中获取未稀释的玻璃体样本。同时还获得了静脉血样。使用灵敏的高效液相色谱测定氨基酸浓度，并通过定量酶联免疫吸附测定测定VEGF水平。采用分光光度法测定血液和玻璃体中的血红蛋白浓度。结果：PDR患者玻璃体中GABA水平为29.4+/-7.8μmol/L，显着高于对照组（18.4+/-5.5μmol/L）（P=.004）。 PDR患者玻璃体谷氨酸浓度（24.7+/-14.0 μmol/L）高于对照组（9.1+/-5.1 μmol/L）（P
Objective: To examine the relative levels of gamma-aminobutyric acid (GABA), glutamate, and vascular endothelial growth factor (VEGF) in the vitreous of nondiabetic and diabetic patients.Methods: Undiluted vitreous samples were obtained from 22 patients with proliferative diabetic retinopathy (PDR) and 28 patients without diabetes who underwent pars plana vitrectomy. Simultaneous venous blood samples also were obtained. Amino acid concentrations were determined using sensitive high-performance liquid chromatography, and VEGF levels by quantitative enzyme-linked immunosorbent assay. Hemoglobin concentrations in the blood and vitreous were determined using spectrophotometry.Results: The level of GABA in the vitreous of patients with PDR, 29.4+/-7.8 mu mol/L, was significantly higher than in controls (18.4+/-5.5 mu mol/L) (P=.004). The vitreous concentration of glutamate was higher in patients with PDR (24.7+/-14.0 mu mol/L) compared with controls (9.1+/-5.1 mu mol/L) (P