Identification of the subunit proteins of 10-nm neurofilaments isolated from axoplasm of squid and Myxicola giant axons.
Identification of the subunit proteins of 10-nm neurofilaments isolated from axoplasm of squid and Myxicola giant axons.
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DOI：
10.1083/jcb.82.2.336
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发表时间：
1979-08
影响因子：
7.8
通讯作者：
Kaiserman-Abramof, I R
中科院分区：
文献类型：
--
作者：
Lasek, R J;Krishnan, N;Kaiserman-Abramof, I R
从漏斗粘霉和鱿鱼的巨轴突的轴浆中分离出神经丝。通过不连续蔗糖梯度离心和 Sepharose 4B 上的凝胶过滤对轴浆进行分级。通过电子显微镜监测级分的神经丝。当在螯合剂存在下分离时，Myxicola 的神经丝几乎完全由摩尔重量为 150,000 和 160,000 的蛋白质亚基组成。鱿鱼神经丝含有两种主要蛋白质，摩尔重量分别为200,000和60,000。将这些蛋白质与文献中报道的其他中间丝蛋白质进行比较。
Neurofilaments were isolated from the axoplasm of the giant axons of Myxicola infundibulum and squid. The axoplasm was fractionated by discontinuous sucrose gradient centrifugation and gel filtration on Sepharose 4B. The fractions were monitored for neurofilaments by electron microscopy. When isolated in the presence of chelating agents, the neurofilaments of Myxicola are composed almost entirely of protein subunits with mol wt of 150,000 and 160,000. Squid neurofilaments contain two major proteins with mol wt of 200,000 and 60,000. These proteins are compared with other intermediate filament proteins which have been reported in the literature.