First confirmation of imported dengue virus serotype 2 complete genome in urine from a Chinese traveler returning from India.
First confirmation of imported dengue virus serotype 2 complete genome in urine from a Chinese traveler returning from India.
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DOI：
10.1186/1743-422x-11-56
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发表时间：
2014-03-25
期刊：
影响因子：
4.8
通讯作者：
Hu K
中科院分区：
文献类型：
--
作者：
Ma X;Zhen W;Yang P;Sun X;Nie W;Zhang L;Xu H;Hu K
登革病毒(DENV)是一种由蚊子传播的病毒，有四种血清型。从患者身上收集血清既耗时又费力，对婴儿和儿童来说存在很高的伤害风险。本研究将尿样登革热的基因组和血清学诊断作为一种非侵入性诊断方法。发病第5天采集血清标本，第8天和第18天分别采集血清和尿液标本。DENV-IgM血清学检测结果显示，血清标本DENV阳性。RT-qPCR检测结果显示，第5天采集的血清样品为DENV阳性，而第8天和第18天采集的血清样品为阴性。第8天和第18天采集的尿样为DENV阳性。我们还从尿样(GenBank KF479233)中测序了登革热病毒的全基因组(10723个碱基)。系统发育和流行病学分析的结果表明，该毒株属于DENV-2组，自助值为100%。在本报告中，我们(1)提供了从印度输入到非流行城市中国的第一例登革热病毒感染的证据，(2)调查了与以前的研究相比，在尿样中检测到登革热病毒的阳性时间更长，(3)提供了从浓缩尿样中提取的登革热病毒全基因组的序列结果(4)讨论了如何利用病毒分型结果来管理血清特异性和再次感染旅行相关登革热的风险。
Dengue virus (DENV) is a mosquito-borne virus that has four serotypes. Collection of serum from patients is time- and labor- consuming, and presents a high injury risk for infants and children. The genomic and serological diagnosis of imported dengue fever from a urine sample was used as a non-invasive diagnostic method in this study. A serum sample was collected on disease day 5, and a serum and urine sample were collected on disease day 8 and 18. The results of serological tests for DENV IgM revealed that the serum samples were positive for DENV. The results of RT-qPCR assay revealed that the serum sample collected on day 5 was DENV-positive; however, the serum sample collected on day 8 and 18 were negative for DENV. The urine sample collected on day 8 and 18 were DENV-positive. We also sequenced the complete DENV genome (10723 bp) from the urine sample (GenBank KF479233). The results of phylogenetic and epidemiological analysis indicated strong confirmation that the strain was located within the DENV-2 group with a 100% bootstrap value. In this report, we (1) provided the first evidence of a DENV infection that was imported from India to a non-endemic city of China, (2) investigated the DENV genome detection having a longer timeframe for positive detection in urine sample compared to previous studies, (3) provided the sequence results for the complete DENV-2 genome from a concentrated urine sample (4) discussed how virus-typing results could be used to manage the risk of sero-specific and re-infected travel-associated dengue fever.