Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia.
Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia.
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早期造血祖细胞参与急性早幼粒细胞白血病的证据。
DOI：
10.1093/ajcp/112.6.819
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发表时间：
1999
影响因子：
3.5
通讯作者：
Salhany,KE
中科院分区：
文献类型：
--
作者：
Edwards,RH;Wasik,MA;Finan,J;Rodriguez,R;Moore,J;Kamoun,M;Rennert,H;Bird,J;Nowell,PC;Salhany,KE
急性早幼粒细胞白血病 (APL) 是急性粒细胞白血病的一个亚型，具有典型的形态学、分子和免疫表型特征。先前的免疫表型分析表明，APL 中的白血病细胞通常表达骨髓标记物 CD33 和 CD13，但缺乏早期造血细胞抗原 CD34 和 HLA-DR 的表达。我们通过流式细胞术分析了 APL 的选定免疫表型特征，结果表明 17 例中有 7 例 (41%) 含有显着的 CD34+ 白血病细胞亚群； 2例APL病例中CD34+髓系细胞为主。通过使用荧光激活细胞分选仪-荧光原位杂交方法，我们证实CD34+细胞具有APL的t(15;17)易位特征。通过使用相同的实验方法，CD34+细胞群被分层为原始CD34+CD38-和定型CD34+CD38+细胞亚群；两个子集中的细胞均含有 t(15;17) 易位。了解 APL 可能部分或大部分是 CD34+ 对于正确诊断很重要。此外，CD34+CD38~母细胞中t(15;17)易位的鉴定表明，至少在某些情况下，APL的致白血病突变发生在原始造血细胞内。
Acute promyelocytic leukemia (APL) represents a subtype of acute myeloid leukemia with characteristic morphologic, molecular, and immunophenotypic features. Previous immunophenotypic analyses have shown that leukemic cells in APL typically express the myeloid markers CD33 and CD13 but lack expression of the early hematopoietic cell antigens CD34 and HLA-DR. We analyzed selected immunophenotypic features of APL by flow cytometry and showed that 7 (41%) of 17 cases contained significant subsets of CD34+leukemic cells; CD34+myeloid cells predominated in 2 APL cases. By using a fluorescence-activated cell sorter–fluorescence in situ hybridization approach, we confirmed that the CD34+cells harbored the t(15;17) translocation characteristic of APL. By using the same experimental approach, CD34+populations were stratified into primitive CD34+CD38−and committed CD34+CD38+cell subpopulations; cells in both subsets contained the t(15;17) translocation. The knowledge that APL may be partly or largely CD34+is important for proper diagnosis. Furthermore, identification of the t(15;17) translocation in CD34+CD38~ blasts indicates that, in at least some cases, the leukemogenic mutation in APL occurs within primitive hematopoietic cells.