In vivo MRI visualization of different cell populations labeled with PARACEST agents
In vivo MRI visualization of different cell populations labeled with PARACEST agents
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DOI：
10.1002/mrm.24411
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发表时间：
2013-06-01
影响因子：
3.3
通讯作者：
Aime, Silvio
中科院分区：
文献类型：
--
作者：
Ferrauto, Giuseppe;Castelli, Daniela Delli;Aime, Silvio
传统的T1或t2 mri造影剂不能同时跟踪不同细胞群的分布，因为松弛增强剂的作用是加性的。本文表明，顺磁性化学交换饱和转移剂提供了在体外和体内通过1H-MRI可视化不同细胞群的机会。Yb-和Eu-HPDO3A复合物已分别用于标记小鼠巨噬细胞（J774.A1）和黑色素瘤细胞（B16-F10）。通过选择性照射两种化学交换饱和转移剂的高位移OH共振，可以跟踪这两种细胞类型。这些顺磁化学交换饱和转移剂具有与Gd-HPDO3A （ProHance (R)）相同的稳定性和体内药代动力学特性，具有巨大的临床翻译潜力。Gd-HPDO3A是一种广泛使用的临床批准的MRI药物。《理性医学杂志》，2013。(c) 2012 Wiley期刊有限公司
Conventional T1- or T2-MRI contrast agents do not allow to track the distribution of different cell populations simultaneously because the effects of relaxation enhancers are additive. Herein, it is shown that paramagnetic chemical exchange saturation transfer agents offer the opportunity to visualize different cell populations in vitro and in vivo by 1H-MRI. Yb- and Eu-HPDO3A complexes have been used to label murine macrophages (J774.A1) and melanoma cells (B16-F10), respectively. By selective irradiation of the highly-shifted OH resonances of the two chemical exchange saturation transfer agents, it has been shown that tracking of the two cell types is possible. These PARAmagnetic Chemical Exchange Saturation Transfer agents have a tremendous potential for clinical translation as they share the same stability and in vivo pharmacokinetic properties of Gd-HPDO3A (ProHance (R)), which is a widely used clinically approved MRI agent. Magn Reson Med, 2013. (c) 2012 Wiley Periodicals, Inc.