Preparation and rapid analysis of antibacterial silver, copper and zinc doped sot-gel surfaces
Preparation and rapid analysis of antibacterial silver, copper and zinc doped sot-gel surfaces
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DOI：
10.1016/j.colsurfb.2012.01.035
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发表时间：
2012-06-01
影响因子：
5.8
通讯作者：
Duffy, Brendan
中科院分区：
文献类型：
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作者：
Jaiswal, Swarna;McHale, Patrick;Duffy, Brendan
临床和工业表面的微生物（包括耐药菌株）定植促进了对有效抗菌和抗菌涂层开发的研究。制备了金属硝酸盐（Ag、Cu、Zn）掺杂的甲基三乙氧基硅烷（MTEOS）涂层，并采用聚丙烯微量滴定板对其抗菌活性进行了快速评价。用不同体积的液体溶胶-凝胶涂覆微量滴定板威尔斯孔，并在各种条件下固化。通过热重分析（TGA）和目视检查来分析固化参数。最佳固化条件确定为50-70 ℃，使用200 μ l的体积。用革兰氏阳性和革兰氏阴性细菌培养物（包括生物膜形成和耐药性菌株）挑战包被的威尔斯孔。在相同浓度下，金属掺杂溶胶-凝胶的抗菌活性顺序为：银>锌>铜。该顺序是由于几个因素造成的，包括通过X射线光电子能谱法测定的溶胶-凝胶涂层表面存在的银纳米颗粒增加，导致通过电感耦合等离子体原子发射光谱法（ICP-AES）测量的更高的洗脱速率。微量滴定板的使用使得能够在相对短的时间内筛选各种溶胶-凝胶涂层对各种细菌的抗菌活性。银掺杂溶胶-凝胶的广谱抗菌活性显示了其作为生物材料涂层的潜力。(C)2012爱思唯尔有限公司版权所有。
The colonisation of clinical and industrial surfaces with microorganisms, including antibiotic-resistant strains, has promoted increased research into the development of effective antibacterial and antifouling coatings. This study describes the preparation of metal nitrate (Ag, Cu, Zn) doped methyltriethoxysilane (MTEOS) coatings and the rapid assessment of their antibacterial activity using polypropylene microtitre plates. Microtitre plate wells were coated with different volumes of liquid sol-gel and cured under various conditions. Curing parameters were analysed by thermogravimetric analysis (TGA) and visual examination. The optimum curing conditions were determined to be 50-70 degrees C using a volume of 200 mu l. The coated wells were challenged with Gram-positive and Gram-negative bacterial cultures, including biofilm-forming and antibiotic-resistant strains. The antibacterial activities of the metal doped sol-gel, at equivalent concentrations, were found to have the following order: silver > zinc > copper. The order is due to several factors, including the increased presence of silver nanoparticles at the sol-gel coating surface, as determined by X-ray photoelectron spectroscopy, leading to higher elution rates as measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The use of microtitre plates enabled a variety of sol-gel coatings to be screened for their antibacterial activity against a wide range of bacteria in a relatively short time. The broad-spectrum antibacterial activity of the silver doped sol-gel showed its potential for use as a coating for biomaterials. (C) 2012 Elsevier B.V. All rights reserved.