Effects of passive immunization against inhibin-peptide on secretion of follicle-stimulating hormone and ovulation rate in ewes carrying the Booroola fecundity gene.
Effects of passive immunization against inhibin-peptide on secretion of follicle-stimulating hormone and ovulation rate in ewes carrying the Booroola fecundity gene.
复制标题
抑制素肽被动免疫对携带 Booroola 繁殖力基因的母羊促卵泡激素分泌和排卵率的影响。
DOI：
10.1095/biolreprod55.6.1351
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发表时间：
1996
影响因子：
3.6
通讯作者：
R. L. Meyer
中科院分区：
文献类型：
--
作者：
J. E. Wheaton;D. Thomas;N. Kusina;R. G. Gottfredson;R. L. Meyer
本研究的目的是确定1)携带Booroola FECB基因的绵羊是否减少了对FSH分泌的抑制素负反馈调节，以及2)不同的FSH分泌是排卵率(OR)基因特异性差异表达的必要条件。方法是比较有和没有FECB基因副本的绵羊对抑制素被动免疫中和的FSH和排卵反应。28只2-3岁的母羊被分配到抗体(α-IF-Ab)或对照组的基因型中。其中3/4的朗布依勒母羊×1/4的Booroola母羊携带1个FECB基因(FECB+；57 kg)和3/4的Rambouillet×1/4的Booroola母羊(++；59 kg)。在繁殖季节使用孕酮释放罐(CIDR-G)同步发情。在-48h单次注射α-If-Ab或对照溶液，针对与绵羊抑制素α亚基N-末端区域匹配的合成抑制素片段制备了Alpha-If-Ab。注射用从绵羊免疫血清中沉淀出α-IF-Ab并进行浓缩。CIDR-G停药后14天，每隔6小时采血一次，时间为-48~48小时。所有母羊都表现出发情和排卵。基因型和α-IF-Ab处理对发情间隔时间没有影响。这两个因素均影响OR(p<；OR=0.001)。对照组++和FECB+母羊的平均OR值分别为1.6和2.7，α-IF-Ab处理++和FECB+母羊的平均OR值分别为2.5和4.6。注射α-IF-Ab后，FSH浓度在6h内升高，12-18h达到峰值，然后下降。++和FECB+母羊的FSH增加幅度相似(分别比对照组高70%和85%)。结果表明：1)抑制素负反馈调节FSH分泌不是FECB基因作用的部位；2)FECB基因增加OR的机制不一定与排卵前卵泡FSH分泌增加有关。
Objectives of the study were to determine whether 1) inhibin negative feedback regulation of FSH secretion is diminished in ewes carrying a copy of the Booroola fecundity (FecB) gene and 2) differential FSH secretion is obligatory for expression of gene-specific differences in ovulation rate (OR). The approach was to compare FSH and ovulatory responses to passive immunoneutralization of inhibin in ewes with and without a copy of the FecB gene. Twenty-eight 2- to 3-yr-old ewes were assigned within genotype to antibody (alpha-IF-Ab) or control groups. Genotypes consisted of 3/4 Rambouillet x 1/4 Booroola ewes with one copy of the FecB gene (FecB+; 57 kg) and 3/4 Rambouillet x 1/4 Booroola ewes without the FecB gene (++; 59 kg). Estrus was synchronized during the breeding season using progesterone-releasing pessaries (CIDR-G). Pessaries were removed at 0 h. A single injection of alpha-IF-Ab or control solution was given at -48 h. Alpha-IF-Ab had been developed against a synthetic inhibin fragment matching the N-terminal region of ovine inhibin's alpha subunit. For injection, alpha-IF-Ab had been precipitated from ovine immune sera and concentrated. Blood samples were collected at 6-h intervals from -48 to 48 h, and laparoscopy was performed 14 days after CIDR-G withdrawal. All ewes exhibited estrus and ovulated. Genotype and alpha-IF-Ab treatment were without effect on intervals to estrus. Both factors affected OR (p < or = 0.001). Mean OR in control ++ and FecB+ ewes were 1.6 and 2.7, respectively; mean OR in alpha-IF-Ab-treated ++ and FecB+ ewes were 2.5 and 4.6, respectively. Following injection of alpha-IF-Ab, FSH concentrations increased within 6 h, peaked 12-18 h later, and then declined. Magnitude of FSH increases was similar in ++ and FecB+ ewes (70% and 85% over control values, respectively). Results demonstrate that 1) inhibin negative feedback regulation of FSH secretion is not a site of FecB gene action and 2) the mechanism by which the FecB gene increases OR does not necessarily involve increased FSH secretion during the period of preovulatory follicular development.