A sensation for inflation: initial swim bladder inflation in larval zebrafish is mediated by the mechanosensory lateral line.
A sensation for inflation: initial swim bladder inflation in larval zebrafish is mediated by the mechanosensory lateral line.
复制标题
膨胀的感觉：斑马鱼幼体的初始鱼鳔膨胀是由机械感觉侧线介导的。
DOI：
10.1101/2023.01.12.523756
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Erickson,Timothy
中科院分区：
文献类型：
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作者：
Venuto,Alexandra;Thibodeau-Beganny,Stacey;Trapani,JosefG;Erickson,Timothy
斑马鱼幼鱼通过游到水面并通过嘴吸入空气使其鱼鳔膨胀来实现中性浮力。我们将这种行为定义为“表面”。人们对这种未被充分认识的幼鱼行为的感官基础知之甚少。一个强有力的候选者是mechanosensory侧线，这是一种基于毛细胞的感觉系统，可以检测来自水流、捕食者、猎物和表面波等来源的流体动力学信息。然而，侧线在介导鱼鳔初始膨胀中的作用尚未报道。为了探索侧线和表面之间的联系，我们使用了一种遗传突变体（lhfpl 5 b −/−），使斑马鱼侧线对机械刺激不敏感。我们观察到，这些侧线突变体中约有一半在最初的膨胀过程中过度膨胀他们的鱼鳔，并成为积极的浮力。因此，我们假设，幼斑马鱼使用他们的侧线温和的相互作用与空气-水界面在浮出水面，以调节鱼鳔膨胀。为了检验侧线缺陷是导致鱼鳔过度膨胀的假设，我们证明了过度膨胀表型需要外源性空气，而转基因毛细胞功能的拯救恢复了正常的膨胀。我们还发现，化学消融前侧线毛细胞在野生型幼虫引起过度膨胀。此外，我们表明，操纵侧线感觉信息的结果在异常膨胀。最后，我们报告野生型和侧线突变幼虫的浮出水面的行为之间的空间和时间差异。总之，我们提出了一种新的感官基础，实现中性浮力，幼斑马鱼用他们的侧线感觉空气-水界面和调节初始鱼鳔膨胀。
Larval zebrafish achieve neutral buoyancy by swimming up to the surface and taking in air through their mouths to inflate their swim bladders. We define this behavior as ‘surfacing’. Little is known about the sensory basis for this underappreciated behavior of larval fish. A strong candidate is the mechanosensory lateral line, a hair cell-based sensory system that detects hydrodynamic information from sources such as water currents, predators, prey and surface waves. However, a role for the lateral line in mediating initial inflation of the swim bladder has not been reported. To explore the connection between the lateral line and surfacing, we used a genetic mutant (lhfpl5b−/−) that renders the zebrafish lateral line insensitive to mechanical stimuli. We observed that approximately half of these lateral line mutants over-inflate their swim bladders during initial inflation and become positively buoyant. Thus, we hypothesized that larval zebrafish use their lateral line to moderate interactions with the air–water interface during surfacing to regulate swim bladder inflation. To test the hypothesis that lateral line defects are responsible for swim bladder over-inflation, we showed that exogenous air is required for the hyperinflation phenotype and transgenic rescue of hair cell function restores normal inflation. We also found that chemical ablation of anterior lateral line hair cells in wild-type larvae causes hyperinflation. Furthermore, we show that manipulation of lateral line sensory information results in abnormal inflation. Finally, we report spatial and temporal differences in the surfacing behavior between wild-type and lateral line mutant larvae. In summary, we propose a novel sensory basis for achieving neutral buoyancy where larval zebrafish use their lateral line to sense the air–water interface and regulate initial swim bladder inflation.