Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood
Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood
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DOI：
10.1111/j.1348-0421.2008.00048.x
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发表时间：
2008-07-01
影响因子：
2.6
通讯作者：
Hirakawa, Hideki
中科院分区：
文献类型：
--
作者：
Moriyama, Kosei;Ando, Chie;Hirakawa, Hideki
在涉及30个循环的条件下，通过PCR在两名健康个体的血液样本中检测到细菌16 S核糖体RNA基因（rDNA），阴性对照盐水未产生任何可见产物。即使从对照样品，涉及35-40个循环的PCR也产生可见条带。在血液样品中检测到的而在对照中未检测到的主要克隆是水生杆菌亚群、寡养单胞菌亚群、布氏杆菌亚群、沙雷氏菌亚群、芽孢杆菌亚群和黄杆菌亚群。没有克隆位于类杆菌-梭菌-大肠杆菌群，这是固有的胃肠道植物群。
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.