A novel transgenic line using the Cre-lox system to allow permanent lineage-labeling of the zebrafish neural crest
A novel transgenic line using the Cre-lox system to allow permanent lineage-labeling of the zebrafish neural crest
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DOI：
10.1002/dvg.22033
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发表时间：
2012-10-01
期刊：
影响因子：
1.5
通讯作者：
Kelsh, Robert N.
中科院分区：
文献类型：
--
作者：
Rodrigues, Frederico S. L. M.;Doughton, Gail;Kelsh, Robert N.
准确的谱系追踪对于理解发育和干细胞生物学至关重要，但对于像神经脊(NC)这样的瞬时和高度分散的细胞类型来说，尤其具有挑战性。本文报道了一个新的斑马鱼转基因品系Tg(-4725sox10：Cre)ba74。该品系在Sox10启动子的特定部分的控制下表达Cre，并且通过与一个Floxed报告品系杂交，作者在本文中表明该品系的表达与使用相同启动子的GFP报告品系的表达是一致的。在与早期表达结构域一致的模式中很容易检测到报告基因的表达。因此，作者看到了NC来源的细胞类型的所有主要群体(色素、神经和骨骼)，以及来自Sox10表达的已知非NC位置的细胞类型，包括耳廓上皮和少突胶质细胞。为斑马鱼NC发育和NC来源干细胞以及耳囊和少突胶质细胞的进一步研究提供了宝贵的工具。创世纪50：750757,2012年。(C)2012年威利期刊公司。
Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but is particularly challenging for transient and highly dispersive cell-types like the neural crest (NC). The authors report in this article a new zebrafish transgenic line Tg(-4725sox10:Cre)ba74. This line expresses Cre under the control of a well-characterized portion of the sox10 promoter and, by crossing to a floxed-reporter line, the authors show in this article that expression in this line is consistent with those described for GFP reporter lines using the same promoter. Reporter expression is readily detected in patterns consistent with the early expression domains. Thus, the authors see all major groups (pigment, neural, and skeletal) of NC-derived cell-types, as well as cell-types derived from the known non-NC sites of sox10 expression, including otic epithelium and oligodendrocytes. This line provides an invaluable tool for the further study of zebrafish NC development and NC-derived stem cells as well as that of the otic vesicle and oligodendrocytes. genesis 50:750757, 2012. (c) 2012 Wiley Periodicals, Inc.