Detection and genetic characterization of cosavirus in a pediatric patient with diarrhea
Detection and genetic characterization of cosavirus in a pediatric patient with diarrhea
复制标题
DOI：
10.1007/s00705-014-2091-6
复制
发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Maneekarn, Niwat
中科院分区：
文献类型：
--
作者：
Khamrin, Pattara;Maneekarn, Niwat
人柯萨奇病毒（humancosavirus，HCoSV）是一种新发现的小核糖核酸病毒科病毒。2010-2011年，从泰国清迈因急性胃肠炎症状入院的儿童中收集了共计411份粪便标本，并通过RT-nested PCR方法筛查HCoSV。在从一名3岁男孩身上采集的一份标本（CMH-N199-11）中发现HCoSV。这是泰国首例儿童腹泻患者感染HCoSV的报告。该病毒与中国HCoSV-A参考株SH 1最相似，属于A6基因型。这些数据表明，在泰国和中国检测到的HCoSV具有相同的进化祖先。我们的研究结果强调需要进一步研究，以了解HCoSV的分布，遗传多样性和人类急性胃肠炎的关联。
Human cosavirus (HCoSV) is a newly discovered virus of the family Picornaviridae. A total of 411 fecal specimens were collected from children admitted to hospitals with acute gastroenteritis symptoms in Chiang Mai, Thailand, in 2010-2011 and screened for HCoSV by an RT-nested PCR method. HCoSV was found in a single specimen (CMH-N199-11) collected from a 3-year-old boy. This represents the first report of HCoSV infection in a pediatric patient with diarrhea in Thailand. Analysis of the complete coding sequence revealed that this HCoSV was most similar to the Chinese HCoSV-A reference strain SH1, and belonged to genotype A6. The data imply that HCoSVs detected in Thailand and China share the same evolutionary ancestor. Our results emphasize the need for further research to understand the distribution, genetic diversity, and association of the HCoSV with acute gastroenteritis in humans.