Tools for live imaging of active Rho GTPases in Xenopus.
Tools for live imaging of active Rho GTPases in Xenopus.
复制标题
用于非洲爪蟾中活性 Rho GTPases 实时成像的工具。
DOI：
10.1002/dvg.22998
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Miller,AnnL
中科院分区：
文献类型：
--
作者：
Stephenson,RachelE;Miller,AnnL
Rho家族GTP酶是在多种细胞过程中协调细胞骨架动力学的信号分子。因为它们仅在其活性（GTP结合）构象中影响细胞骨架的局部变化，所以在空间和时间上监测Rho GTP酶的活性状态的能力对于理解它们的功能至关重要。在这里，我们总结了流行的工具用于活性Rho GTP酶的实时成像，概述了这些方法的优点和缺点。此外，我们强调了非洲爪蟾胚胎的关键特征，使其非常适合于上皮细胞生物学，并讨论了Rho活性报告基因在非洲爪蟾胚胎中的应用如何导致发现一种新的现象，连接Rho耀斑。
Rho family GTPases are signaling molecules that orchestrate cytoskeletal dynamics in a variety of cellular processes. Because they effect localized changes to the cytoskeleton only in their active (GTP‐bound) conformation, the ability to monitor the active state of Rho GTPases in space and time is critical for understanding their function. Here, we summarize popular tools used for live imaging of active Rho GTPases, outlining advantages and drawbacks of these approaches. Additionally, we highlight key features of theXenopus laevisembryo that make it well‐suited for epithelial cell biology and discuss how application of Rho activity reporters in theXenopus laevisembryo led to the discovery of a novel phenomenon, junctional Rho flares.