Methylation of multiple genes as a candidate biomarker in non-small cell lung cancer
Methylation of multiple genes as a candidate biomarker in non-small cell lung cancer
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DOI：
10.1016/j.canlet.2010.12.011
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发表时间：
2011-04-01
期刊：
影响因子：
9.7
通讯作者：
Chen, Longbang
中科院分区：
文献类型：
--
作者：
Zhang, Youwei;Wang, Rui;Chen, Longbang
DNA甲基化异常是人类肿瘤中常见的现象。本研究的目的是调查中国人群中非小细胞肺癌（NSCLC）的甲基化谱。采用甲基化特异性PCR检测78例配对NSCLC标本及癌旁正常组织、110例I/II期NSCLC和50例无癌血浆中20个肿瘤抑制基因（TSGs）的甲基化状态。结果显示，APC、CDH 13、KLK 10、DLEC 1、RASSF 1A、EFEMP 1、SFRP 1、RAR β和p16（INK 4A）9个基因在NSCLC中的甲基化频率显著高于正常组织（P
Aberrant DNA methylation is a common phenomenon in human cancer. The aims of this study were to investigate the methylation profiles of non-small cell lung cancer (NSCLC) in the Chinese population. Twenty tumor suppressor genes (TSGs) were determined of the methylation status using methylation-specific PCR in 78 paired NSCLC specimens and adjacent normal tissues, as well as in 110 Stage I/II NSCLC and 50 cancer-free plasmas. The results showed that, nine genes (APC, CDH13, KLK10, DLEC1, RASSF1A, EFEMP1, SFRP1, RAR beta and p16(INK4A)) demonstrated a significantly higher frequency of methylation in NSCLC compared with the normal tissues (P