Comparative rates of proteoglycan synthesis and size of proteoglycans in normal and osteoarthritic chondrocytes.
Comparative rates of proteoglycan synthesis and size of proteoglycans in normal and osteoarthritic chondrocytes.
复制标题
正常软骨细胞和骨关节炎软骨细胞中蛋白多糖合成率和蛋白多糖大小的比较。
DOI：
10.1002/art.1780261009
复制
发表时间：
1983
影响因子：
--
通讯作者：
Mankin,HJ
中科院分区：
文献类型：
--
作者：
Teshima,R;Treadwell,BV;Trahan,CA;Mankin,HJ
用梭菌胶原酶从软骨中分离正常人和骨关节炎细胞。细胞在含35SO_4的培养基中悬浮培养。中度组织学分级（4-8级）的骨关节炎软骨产生的软骨细胞对35SO_4的吸收率是正常软骨细胞的3-4倍。然而，从疾病的轻度（0-3级）或更晚期（9-13级）阶段分离的软骨细胞的掺入率降低到正常水平。这些结果证实了早期使用器官培养物的研究中获得的结果，并表明当骨关节炎细胞从其基质环境中分离时，它们继续以增加的速率合成大分子。对分离细胞合成物质的分析表明，含~（35）SO_4分子的大小与疾病的严重程度成反比。
Normal human and osteoarthritic cells were isolated from cartilage with clostridial collagenase. The cells were grown in media as a suspension culture in the presence of35SO4.Osteoarthritic cartilage of moderate histologic grade (4–8) yielded chondrocytes which incorporated35SO4at a rate 3–4 times greater than did normal chondrocytes. The rate of incorporation, however, decreased to normal levels with chondrocytes isolated from mild (grade 0–3) or more advanced (grade 9–13) stages of the disease. These results corroborate those obtained in earlier studies using organ cultures and show that when osteoarthritic cells are isolated from their matrix environment, they continue to synthesize macromolecules at an increased rate. Analysis of the material synthesized by the isolated cells on sizing column demonstrated an inverse relationship between the size of the35SO4containing molecules and the severity of the disease.