ALTERATIONS IN PLASMA-LIPOPROTEINS AND APOLIPOPROTEINS ASSOCIATED WITH ESTROGEN-INDUCED HYPERLIPIDEMIA IN THE LAYING HEN
ALTERATIONS IN PLASMA-LIPOPROTEINS AND APOLIPOPROTEINS ASSOCIATED WITH ESTROGEN-INDUCED HYPERLIPIDEMIA IN THE LAYING HEN
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DOI：
10.1111/j.1432-1033.1989.tb14996.x
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发表时间：
1989-09-01
期刊：
影响因子：
--
通讯作者：
CHAPMAN, MJ
中科院分区：
文献类型：
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作者：
HERMIER, D;FORGEZ, P;CHAPMAN, MJ
蛋鸡是一种可以评估雌激素在脂质转运中的作用机制的生理模型。采用等密度梯度超离心法对蛋鸡血浆脂蛋白进行分离，并对各亚组分的理化性质和载脂蛋白含量进行测定。然后将这种雌激素特异性特征的定性和定量方面与未成熟鸡的特征进行比较。如前所述，雌激素诱导极低密度脂蛋白（VLDL）急剧升高（高达900 mg/dl）。事实上，密度高达1.035 g/ml的富含甘油三酯的脂蛋白，即VLDL及其残余物，表现为一个连续体，其大小（20.5-21 nm），电泳迁移率（. β样）和载脂蛋白含量变化不大；apo B-100 （540 kDa）占主导地位，apo A-I (27 kDa), apo VLDL-II （19 kDa）和apo c样蛋白（13 kDa）作为次要成分存在。未成熟鸡体内典型的高密度脂蛋白（HDL）被理化性质截然不同的脂蛋白群体所取代。因此，在1.030 ~ 1.158 g/ml的密度范围内，这些颗粒呈单一的不对称峰分布，其分布范围与载脂蛋白- β的分布范围重叠。-富粒子在其下限。.rho。在未成熟的鸟类中，存在1.030-1.158 g/ml的脂蛋白（& 200 mg/dl），比HDL低两到三倍。此外，它们表现出与低密度脂蛋白（LDL）和高密度脂蛋白（HDL）相同的物理和化学特性，并且在表现β方面与低密度脂蛋白相似。但在尺寸上与hld相似（9- 15nm直径）。它们的蛋白质片段在载脂蛋白A-I的主要含量上也与高密度脂蛋白相似；少量载脂蛋白VLDL-II和载脂蛋白c样蛋白也被检测到。在。rho处发现了大量的脂质。> 1.195 g/ml：这些物质在未成熟的鸡中不存在，可能反映了卵黄蛋白原的存在。因此，蛋鸡的高雌激素状态与脂蛋白和载脂蛋白谱的显著改变有关。这种调节可能与涉及雌激素诱导的高脂血症的临床疾病有关。
The laying hen represents a physiological model in which the mechanisms of action of estrogens of lipid transport can be evaluated. The plasma lipoproteins in the laying hen were subfractionated into discrete particle species by isopycnic density graident ultracentrifugation and the physicochemical properties and apolipoprotein contents of individual subfractions evaluated. The qualitative and quantitive aspects of this estrogen-specific profile were then compared to those of the immature chicken. As observed earlier, estrogens induced dramatic elevation in very-low-density lipoproteins (VLDL) (up to 900 mg/dl). Indeed, triglyceride-rich lipoproteins with densities up to 1.035 g/ml, i.e. VLDL and their remnants, behaved as a continuum which displayed little variation in size (20.5-21 nm), electrophoretic mobility (.beta.-like) and apolipoprotein content; apo B-100 (540 kDa) predominated while apo A-I (27 kDa), apo VLDL-II (19 kDA) and an apo-C-like protein (13 kDa) were present as minor components. The typical high-density lipoproteins (HDL) in the immature chicken were replaced by a lipoprotein population whose physicochemical properties were quite distinct. Thus these particles were distributed as a single, asymmetric peak over the density range 1.030-1.158 g/ml, a wide interval which overlapped that of apo-.beta.-rich particles at its lower limit. The .rho. 1.030-1.158 g/ml lipoproteins were present at concentrations (& 200 mg/dl) some twofold to threefold lower than those of HDL in immature birds. Furthermore, they displayed physical and chemical properties in common with both low-density lipoproteins (LDL) and HDL and were LDL-like in exhibiting .beta. mobility but HLD-like in size (9-15 nm diameter). Their protein moiety was also HDL-like in its predominant content of apo A-I; small amounts of apo VLDL-II and the apo-C-like protein were also detected. Substantial amounts of lipid were found at .rho. > 1.195 g/ml: such substances are absent in the immature chicken and may reflect the presence of vitellogenins. The hyperestrogenic state in the laying hen is therefore associated with mauor modifications in lipoprotein and apolipoprotein profile. Such modications may be of relevance to clinical disorders invovling estrogen-induced hyperlipidemia.