3D timelapse analysis of muscle satellite cell motility.
3D timelapse analysis of muscle satellite cell motility.
复制标题
DOI：
10.1002/stem.178
复制
发表时间：
2009-10
期刊：
影响因子：
5.2
通讯作者：
Cornelison, D. D. W.
中科院分区：
文献类型：
--
作者：
Siegel, Ashley L.;Atchison, Kevin;Fisher, Kevin E.;Davis, George E.;Cornelison, D. D. W.
骨骼肌修复和再生需要卫星细胞的活性，卫星细胞是散布在整个组织中的一群肌源干细胞，并被激活以增殖和分化以响应肌外伤或疾病。虽然卫星细胞似乎需要导航局部肌肉组织才能到达受损区域，但有关这种运动的数据相对较少，而且大多数研究都是针对永生化细胞系。我们发现，原代卫星细胞比成肌细胞系更具运动性，并且与层粘连蛋白的粘附促进原代细胞运动性是其他基质的四倍以上。使用延时视频显微镜评估单个活肌纤维上的卫星细胞运动，我们确定了卫星细胞运动中层粘连蛋白结合整合素α7β1的需求，以及肝细胞生长因子在促进定向持久性中的作用。驻留在肌纤维上的卫星细胞的广泛迁移行为表明，在根据固定样本确定相邻细胞是否来自同一母细胞的子细胞时要小心。我们还观察到单个卫星细胞之间比之前假设的更持久的长期接触、潜在的细胞间吸引和排斥相互作用以及宿主肌纤维之间的迁移。基于此类活动，我们分析了“寻路”线索的表达，发现卫星细胞表达多种引导配体和受体。总之，这些数据表明卫星细胞在体内的迁移可能比目前认为的更广泛，并且可以通过信号组合来调节，包括趋粘性、可溶性因子和引导信号。干细胞 2009；27：2527–2538
Skeletal muscle repair and regeneration requires the activity of satellite cells, a population of myogenic stem cells scattered throughout the tissue and activated to proliferate and differentiate in response to myotrauma or disease. While it seems likely that satellite cells would need to navigate local muscle tissue to reach damaged areas, relatively little data on such motility exist, and most studies have been with immortalized cell lines. We find that primary satellite cells are significantly more motile than myoblast cell lines, and that adhesion to laminin promotes primary cell motility more than fourfold over other substrates. Using timelapse videomicroscopy to assess satellite cell motility on single living myofibers, we have identified a requirement for the laminin-binding integrin α7β1 in satellite cell motility, as well as a role for hepatocyte growth factor in promoting directional persistence. The extensive migratory behavior of satellite cells resident on muscle fibers suggests caution when determining, based on fixed specimens, whether adjacent cells are daughters from the same mother cell. We also observed more persistent long-term contact between individual satellite cells than has been previously supposed, potential cell-cell attractive and repulsive interactions, and migration between host myofibers. Based on such activity, we assayed for expression of “pathfinding” cues, and found that satellite cells express multiple guidance ligands and receptors. Together, these data suggest that satellite cell migration in vivo may be more extensive than currently thought, and could be regulated by combinations of signals, including adhesive haptotaxis, soluble factors, and guidance cues. Stem Cells 2009;27:2527–2538
登录
查看更多内容
影响因子：
7.8
作者：
Belkin, AM;Zhidkova, NI;Balzac, F;Altruda, F;Tomatis, D;Maier, A;Tarone, G;Koteliansky, VE;Burridge, K
通讯作者：
Burridge, K
影响因子：
37.3
作者：
Fisher KE;Pop A;Koh W;Anthis NJ;Saunders WB;Davis GE
通讯作者：
Davis GE
影响因子：
4
作者：
Humphries JD;Byron A;Humphries MJ
通讯作者：
Humphries MJ
影响因子：
2.7
作者：
Cornelison, DDW;Olwin, BB;Wold, BJ
通讯作者：
Wold, BJ
影响因子：
11.8
作者：
Conboy, IM;Rando, TA
通讯作者：
Rando, TA